Rick van Leeuwen
Rick van Leeuwen is senior-tchnician/laboratory manager of the Department of Cardiology at Maastricht. He has more than 25 years of expertise as senior technician in different research labs at different locations in the Netherlands, of which 17 yeart at Maastricht UMC+.
An important general aspect of his work is to manage all the cardiology research laboratory facilities, control the financial status of the bench fee of the research projects and update lab permits for internal and external audits.
My research aspect is more in the protein field, like unravelling protein-protein interactions in viral myocarditis with techniques like pull down assays and co-immunoprecipitation or to identify new protein targets by mass spectrometry. In order to understand the mechanism, I study in vitro, signal transduction pathways in isolated neonatal cardiomyocytes and fibroblasts by Western Blotting. Therefore, I use also other primary cells (macrophages), isolation of adult cardiomyocytes and cell lines.
For micro-RNA research, I use Northern Blot techniques and to study mRNA targets or unknown microRNA I perform RNA-immunoprecipitation (RIP) assays with Argonaute-2 and RNA pull down assay. For establishing the targets, I use next gen RNA sequencing tools. Furthermore, I am introducing novel techniques/technologies to optimise our research scoops and to improve routine lab techniques with recent high tech compounds.
For education purposes, I support the graduate students in their research.