Stefan (SR) Reinhold

MSc
Birthdate: 13-03-1985

Experience

Education:

- M.Sc. in molecular and applied biotechnology, September 2017, RWTH Aachen University
- B.Sc. in Biotechnology, March 2013

Expertise:

- Cell culture; Isolation of Primary mesenchymal stroma-/stem cells from umbilical cord and bone marrow, Cultivation of different cell lines (MEF, L929, Huh7, CHO, Flp-In TREx 293); culture of germline derived pluripotent stem cells (gPSC, mouse) and Differentiation to cardiomyocytes), fermentation of cho dg44
- functional cell culture assays; Scratch Assay, Boyden Chamber Assay
- molecular Analysis; RNA, DNA & Plasmid Isolation, cDNA Synthesis, different PCR methods, primer design, sequence Analysis with vector nti
- Protein analyses; IHC, BCA-Assay, Western Blot (semi-dry), SDS-Page
- Protein purification; Size-exclusion, IMAC (His-tagged Proteins)
- Stainings: Life/Dead Staining, HE Staining, Alizarin Red, Oil red o
- Microbial work; Transformation, Cloning
- Microscopy; fluorescence and light microscopy

Position

Organisations:
CARIM (PhD Student)
Research: