Chris Reutelingsperger


Prof. Chris Reutelingsperger studied Biochemistry at the University of Utrecht. As a PhD student, he discovered and patented the anticoagulant annexin A5 (VAscular Anticoagulant) and defended the thesis 'Vascular anticoagulant, a novel anticoagulant mechanism' at Maastricht University in 1987. He was awarded the five-year Senior Fellowship of the Royal Dutch Academy of Sciences (KNAW) and spent part of his post-doc time at the Boehringer Ingelheim Institute für Arzneimittelforschung in Vienna, to work on elucidating the genetic code of annexin A5.

In 1994, he discovered and patented the power of annexin A5 to detect apoptotic cells. In 1992, he received a tenure track at the Department of Biochemistry of Maastricht University, resulting in a tenured position as Associate Professor in 1997. In 2009, he was appointed Professor of Biochemistry of Apoptosis at Maastricht University. He (co-)authored over 200 peer reviewed papers (H-index 68, source Scholar Google). He is (co-)inventor of 12 granted patents and CSO of PharmaTarget, a biotech company which he co-founded in 2006. He was President of the 8th International Conference on Annexins in 2015. Currently, he chairs the Scientific Advisory Board of Matisse Pharmaceuticals (Netherlands), a biotech company developing therapeutic drugs to treat sepsis.

Chris Reutelingsperger's research activities address molecular and cellular mechanisms of vascular remodeling, vasculitis, atherosclerosis and vascular calcification. His interests focus on vascular calcification, cell death programs, phagocytosis, complement, vitamin K-dependent proteins and annexins, with emphasis on annexins A1 and A5. His major experimental approach utilises in vitrocell biology an in-house developed integrated in vitro assay platform (The BioHybrid, 'organ on-a-chip') covering all major vascular cell types and processes in the pathophysiology of vascular diseases. His strategy encompasses linking basic research to clinical application by establishing collaborations with clinical research and with industry.

Department of Biochemistry
Universiteitssingel 50, 6229 ER Maastricht
PO Box 616, 6200 MD Maastricht
Room number: H4.354
T: +31(0)43 388 16 74

  • 2022
    • Fronza Broering, M., Leão, M. C., da Rocha, G. H. O., Scharf, P. R. S., Fillipi Xavier, L., de Cristo Soares Alves, A., Castro, I., Reutelingsperger, C., Klimuk Uchiyama, M., Araki, K., Guterres, S. S., Pohlmann, A. R., & Farsky, S. H. P. (2022). Development of Annexin A1-surface-functionalized metal-complex multi-wall lipid core nanocapsules and effectiveness on experimental colitis. European Journal of Pharmaceutics and Biopharmaceutics, 181, 49-59.
    • Pavlic, A., Bahram Sangani, N., Kerins, J., Nicolaes, G., Schurgers, L., & Reutelingsperger, C. (2022). Vascular Smooth Muscle Cell Neutral Sphingomyelinase 2 in the Release of Exosomes and Vascular Calcification. International journal of molecular sciences, 23(16), [9178].
    • Oggero, S., Godec, T., van Gorp, R., Pinto, L. A., Schurgers, J. L., Reutelingsperger, C., Sever, P., Norling, L. V., Perretti, M., & Gupta, A. (2022). Role of plasma extracellular vesicles in prediction of cardiovascular risk and alterations in response to statin therapy in hypertensive patients. Journal of Hypertension, 40(8), 1522-1529.
    • Klein, L., Ophelders, D. R. M. G., van den Hove, D., Damoiseaux, M., Rutten, B. P. F., Reutelingsperger, C. P. M., Schurgers, L. J., & Wolfs, T. G. A. M. (2022). Prenatal administration of multipotent adult progenitor cells modulates the systemic and cerebral immune response in an ovine model of chorioamnionitis. Brain, Behavior, & Immunity - Health, 23, [100458].
    • Petsophonsakul, P., Burgmaier, M., Willems, B., Heeneman, S., Stadler, N., Gremse, F., Reith, S., Burgmaier, K., Kahles, F., Marx, N., Natour, E., Bidar, E., Jacobs, M., Mees, B., Reutelingsperger, C., Furmanik, M., & Schurgers, L. (2022). Nicotine promotes vascular calcification via intracellular Ca2+-mediated, Nox5-induced oxidative stress and extracellular vesicle release in vascular smooth muscle cells. Cardiovascular Research, 118(9), 2196-2210.
    • Jaminon, A. M. G., Rapp, N., Akbulut, A. C., Dzhanaev, R., Reutelingsperger, C. P., Jahnen-Dechent, W., & Schurgers, L. J. (2022). A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro. Journal of visualized experiment, (184), [e64029].
    • van Kuijk, K., Demandt, J. A. F., Perales-Patón, J., Theelen, T. L., Kuppe, C., Marsch, E., de Bruijn, J., Jin, H., Gijbels, M. J., Matic, L., Mees, B. M. E., Reutelingsperger, C. P. M., Hedin, U., Biessen, E. A. L., Carmeliet, P., Baker, A. H., Kramann, R. K., Schurgers, L. J., Saez-Rodriguez, J., & Sluimer, J. C. (2022). Deficiency of myeloid PHD proteins aggravates atherogenesis via macrophage apoptosis and paracrine fibrotic signalling. Cardiovascular Research, 118(5), 1232-1246.
    • You, W., Berendschot, T. T. J. M., Knoops, K., van Zandvoort, M. A. M. J., Webers, C. A. B., Reutelingsperger, C. P. M., & Gorgels, T. G. M. F. (2022). Single Cell Analysis of Reversibility of the Cell Death Program in Ethanol-Treated Neuronal PC12 Cells. International journal of molecular sciences, 23(5), [2650].
    • Hrdinova, J., Fernández, D. I., Ercig, B., Tullemans, B. M. E., Suylen, D. P. L., Agten, S. M., Jurk, K., Hackeng, T. M., Vanhoorelbeke, K., Voorberg, J., Reutelingsperger, C. P. M., Wichapong, K., Heemskerk, J. W. M., & Nicolaes, G. A. F. (2022). Structure-Based Cyclic Glycoprotein Ibα-Derived Peptides Interfering with von Willebrand Factor-Binding, Affecting Platelet Aggregation under Shear. International journal of molecular sciences, 23(4), [2046].
    • Amiral, J., Busch, M. H., Timmermans, S. A. M. E. G., Reutelingsperger, C. P., & van Paassen, P. (2022). Development of IgG, IgM, and IgA Autoantibodies Against Angiotensin Converting Enzyme 2 in Patients with COVID-19. Journal of Applied Laboratory Medicine, 7(1), 382-386.
    • Sheikh, M. H., Errede, M., D'Amati, A., Khan, N. Q., Fanti, S., Loiola, R. A., McArthur, S., Purvis, G. S. D., O'Riordan, C. E., Ferorelli, D., Dell'Erba, A., Kieswich, J., Reutelingsperger, C., Maiorano, E., Yaqoob, M., Thiemermann, C., Baragetti, A., Catapano, A. L., Norata, G. D., ... Solito, E. (2022). Impact of metabolic disorders on the structural, functional, and immunological integrity of the blood-brain barrier: Therapeutic avenues. Faseb Journal, 36(1), [e22107].
  • 2021
    • Tobal, R., Potjewijd, J., van Empel, V. P. M., Ysermans, R., Schurgers, L. J., Reutelingsperger, C. P., Damoiseaux, J. G. M. C., & van Paassen, P. (2021). Vascular Remodeling in Pulmonary Arterial Hypertension: The Potential Involvement of Innate and Adaptive Immunity. Frontiers in medicine, 8, 806899. [806899].
    • Ercig, B., Arfman, T., Hrdinova, J., Wichapong, K., Reutelingsperger, C. P. M., Vanhoorelbeke, K., Nicolaes, G. A. F., & Voorberg, J. (2021). Conformational plasticity of ADAMTS13 in hemostasis and autoimmunity. Journal of Biological Chemistry, 297(4), [101132].
    • Sangani, N. B., Gomes, A. R., Curfs, L. M. G., & Reutelingsperger, C. P. (2021). The role of Extracellular Vesicles during CNS development. Progress in Neurobiology, 205, [102124].
    • Chen, J. M., Norling, L. V., Mesa, J. G., Silva, M. D., Burton, S. E., Reutelingsperger, C., Perretti, M., & Cooper, D. (2021). Annexin A1 attenuates cardiac diastolic dysfunction in mice with inflammatory arthritis. Proceedings of the National Academy of Sciences of the United States of America, 118(38), [e2020385118].
    • Huckriede, J., de Vries, F., Hultstroem, M., Wichapong, K., Reutelingsperger, C., Lipcsey, M., de Frutos, P. G., Frithiof, R., & Nicolaes, G. A. F. (2021). Histone H3 Cleavage in Severe COVID-19 ICU Patients. Frontiers in Cellular and Infection Microbiology, 11, [694186].
    • van Gorp, R. H., Baaten, C. C. F. M. J., Habibi, A., Jaminon, A. M. G., Peeters, F. E. C. M., Leenders, P., Crijns, H. J. G. M. C., Heemskerk, J. W. M., Reutelingsperger, C. P., Spronk, H. M., & Schurgers, L. J. (2021). Vitamin K antagonist use induces calcification and atherosclerotic plaque progression resulting in increased hypercoagulability. European heart journal open, 1(2), [oeab017].
    • Huckriede, J., Anderberg, S. B., Morales, A., de Vries, F., Hultstrom, M., Bergqvist, A., Ortiz-Perez, J. T., Sels, J. W., Wichapong, K., Lipcsey, M., van de Poll, M., Larsson, A., Luther, T., Reutelingsperger, C., Garcia de Frutos, P., Frithiof, R., & Nicolaes, G. A. F. (2021). Evolution of NETosis markers and DAMPs have prognostic value in critically ill COVID-19 patients. Scientific Reports, 11(1), [15701].
    • Jaminon, A. M. G., Akbulut, A. C., Rapp, N., Kramann, R., Biessen, E. A. L., Temmerman, L., Mees, B., Brandenburg, V., Dzhanaev, R., Jahnen-Dechent, W., Floege, J., Uitto, J., Reutelingsperger, C. P., & Schurgers, L. J. (2021). Development of the BioHybrid Assay: Combining Primary Human Vascular Smooth Muscle Cells and Blood to Measure Vascular Calcification Propensity. Cells, 10(8), [2097].
    • Liu, X., Arfman, T., Wichapong, K., Reutelingsperger, C. P. M., Voorberg, J., & Nicolaes, G. A. F. (2021). PAD4 takes charge during neutrophil activation: Impact of PAD4 mediated NET formation on immune-mediated disease. Journal of Thrombosis and Haemostasis, 19(7), 1607-1617.
    • Ercig, B., Gomes Graca, N. A., Kangro, K., Arfman, T., Wichapong, K., Hrdinova, J., Kaijen, P., van Alphen, F., van den Biggelaar, M., Vanhoorelbeke, K., Veyradier, A., Coppo, P., Reutelingsperger, C., Nicolaes, G. A. F., Männik, A., & Voorberg, J. (2021). N-glycan mediated shielding of ADAMTS13 prevents binding of pathogenic autoantibodies in immune-mediated TTP. Blood, 137(19), 2694-2698.
    • Ries, M., Watts, H., Mota, B. C., Lopez, M. Y., Donat, C. K., Baxan, N., Pickering, J. A., Chau, T. W., Semmler, A., Gurung, B., Aleksynas, R., Abelleira-Hervas, L., Iqbal, S. J., Romero-Molina, C., Hernandez-Mir, G., D'Amati, A., Reutelingsperger, C., Goldfinger, M. H., Gentleman, S. M., ... Sastre, M. (2021). Annexin A1 restores cerebrovascular integrity concomitant with reduced amyloid-β and tau pathology. Brain, 144, 1526-1541.
    • Liu, X., Wichapong, K., Lamers, S., Reutelingsperger, C. P. M., & Nicolaes, G. A. F. (2021). Autocitrullination of PAD4 does not alter its enzymatic activity: In vitro and in silico studies. International Journal of Biochemistry & Cell Biology, 134, 105938. [105938].
    • van Gorp, R. H., Dijkgraaf, I., Bröker, V., Bauwens, M., Leenders, P., Jennen, D., Dweck, M. R., Bucerius, J., Briedé, J. J., van Ryn, J., Brandenburg, V., Mottaghy, F., Spronk, H. M. H., Reutelingsperger, C. P., & Schurgers, L. J. (2021). Off-target effects of oral anticoagulants - vascular effects of vitamin K antagonist and non-vitamin K antagonist oral anticoagulant dabigatran etexilate. Journal of Thrombosis and Haemostasis, 19(5), 1348-1363.
    • Timmermans, S. A. M. E. G., Damoiseaux, J. G. M. C., Werion, A., Reutelingsperger, C. P., Morelle, J., & van Paassen, P. (2021). Functional and Genetic Landscape of Complement Dysregulation Along the Spectrum of Thrombotic Microangiopathy and its Potential Implications on Clinical Outcomes. Kidney International Reports, 6(4), 1099-1109.
    • Demandt, J. A. F., Dubois, L. J., van Kuijk, K., Zat'ovicova, M., Jin, H., Parkkila, S., van der Laan, S. W., Jelenska, L., Mees, B. M. E., Reutelingsperger, C. P. M., Cleutjens, K. B. J. M., van der Kallen, C. J. H., Schalkwijk, C. G., van Greevenbroek, M. M. J., Biessen, E. A. L., Pasterkamp, G., Pastorekova, S., Stehouwer, C. D. A., & Sluimer, J. C. (2021). The hypoxia-sensor carbonic anhydrase IX affects macrophage metabolism, but is not a suitable biomarker for human cardiovascular disease. Scientific Reports, 11(1), [425].
  • 2020
    • Aendekerk, J. P. I., Timmermans, S. A. M. E. G., Busch, M. H., Potjewijd, J., Heeringa, P., Damoiseaux, J. G. M. C., Reutelingsperger, C. P., van Paassen, P., & Limburg Renal Registry (2020). Urinary Soluble CD163 and Disease Activity in Biopsy-Proven ANCA-Associated Glomerulonephritis. Clinical journal of the American Society of Nephrology, 15(12), 1740-1748.
    • Gomes, A. R., Sangani, N. B., Fernandes, T. G., Diogo, M. M., Curfs, L. M. G., & Reutelingsperger, C. P. (2020). Extracellular Vesicles in CNS Developmental Disorders. International journal of molecular sciences, 21(24), [9428].
    • Busch, M. H., Timmermans, S. A. M. E. G., Nagy, M., Visser, M., Huckriede, J., Aendekerk, J. P., de Vries, F., Potjewijd, J., Jallah, B., Ysermans, R., Oude Lashof, A. M. L., Breedveld, P. H., van de Poll, M. C. G., van de Horst, I. C. C., van Bussel, B. C. T., Theunissen, R. O. M. F. I. H., Spronk, H. M. H., Damoiseaux, J. G. M. C., ten Cate, H., ... van Paassen, P. (2020). Neutrophils and Contact Activation of Coagulation as Potential Drivers of COVID-19. Circulation, 142(18), 1787-1790.
    • Beurskens, D. M. H., Huckriede, J. P., Schrijver, R., Hemker, H. C., Reutelingsperger, C. P., & Nicolaes, G. A. F. (2020). The Anticoagulant and Nonanticoagulant Properties of Heparin. Thrombosis and Haemostasis, 120(10), 1371-1383.
    • Timmermans, S. A. M. E. G., Damoiseaux, J. G. M. C., Reutelingsperger, C. P., Paassen, P. V., & Limburg Renal Registry (2020). More about complement in the antiphospholipid syndrome. Blood, 136(12), 1456-1459.
    • Furmanik, M., Chatrou, M., van Gorp, R., Akbulut, A., Willems, B., Schmidt, H., van Eys, G., Bochaton-Piallat, M-L., Proudfoot, D., Biessen, E., Hedin, U., Perisic, L., Mees, B., Shanahan, C., Reutelingsperger, C., & Schurgers, L. (2020). Reactive Oxygen-Forming Nox5 Links Vascular Smooth Muscle Cell Phenotypic Switching and Extracellular Vesicle-Mediated Vascular Calcification. Circulation Research, 127(7), 911-927.
    • Timmermans, S. A. M. E. G., Werion, A., Spaanderman, M. E. A., Reutelingsperger, C. P., Damoiseaux, J. G. M. C., Morelle, J., & Paassen, P. (2020). The natural course of pregnancies in women with primary atypical haemolytic uraemic syndrome and asymptomatic relatives. British Journal of Haematology, 190(3), 442-449.
    • Bol, M. E., Beurskens, D. M. H., Delnoij, T. S. R., Roekaerts, P. M. H. J., Reutelingsperger, C. P. M., Delhaas, T., van de Poll, M. C. G., Sels, J-W. E. M., & Nicolaes, G. A. F. (2020). Variability of Microcirculatory Measurements in Critically Ill Patients. Shock, 54(1), 9-14.
    • Hebeda, C. B., Sandri, S., Benis, C. M., de Paula-Silva, M., Loiola, R. A., Reutelingsperger, C., Perretti, M., & Farsky, S. H. P. (2020). Annexin A1/Formyl Peptide Receptor Pathway Controls Uterine Receptivity to the Blastocyst. Cells, 9(5), [1188].
    • Beurskens, D. M. H., Bol, M. E., Delhaas, T., van de Poll, M. C. G., Reutelingsperger, C. P. M., Nicolaes, G. A. F., & Sels, J-W. E. M. (2020). Decreased endothelial glycocalyx thickness is an early predictor of mortality in sepsis. Anaesthesia and Intensive Care, 48(3), 221-228.
    • McArthur, S., Juban, G., Gobbetti, T., Desgeorges, T., Theret, M., Gondin, J., Toller-Kawahisa, J. E., Reutelingsperger, C. P., Chazaud, B., Perretti, M., & Mounier, R. (2020). Annexin A1 drives macrophage skewing to accelerate muscle regeneration through AMPK activation. Journal of Clinical Investigation, 130(3), 1156-1167.
    • Baues, M., Klinkhammer, B. M., Ehling, J., Gremse, F., van Zandvoort, M. A. M. J., Reutelingsperger, C. P. M., Daniel, C., Amann, K., Babickova, J., Kiessling, F., Floege, J., Lammers, T., & Boor, P. (2020). A collagen-binding protein enables molecular imaging of kidney fibrosis in vivo. Kidney International, 97(3), 609-614.
    • Timmermans, S. A. M. E. G., Werion, A., Damoiseaux, J. G. M. C., Morelle, J., Reutelingsperger, C. P., & van Paassen, P. (2020). Diagnostic and Risk Factors for Complement Defects in Hypertensive Emergency and Thrombotic Microangiopathy. Hypertension, 75(2), 422-430.
  • 2019
    • Zub, E., Canet, G., Garbelli, R., Blaquiere, M., Rossini, L., Pastori, C., Sheikh, M., Reutelingsperger, C., Klement, W., de Bock, F., Audinat, E., Givalois, L., Solito, E., & Marchi, N. (2019). The GR-ANXA1 pathway is a pathological player and a candidate target in epilepsy. Faseb Journal, 33(12), 13998-14009.
    • Fontaine, M. A. C., Westra, M. M., Bot, I., Jin, H., Franssen, A. J. P. M., Bot, M., de Jager, S. C. A., Dzhagalov, I., He, Y-W., van Vlijmen, B. J. M., Gijbels, M. J. J., Reutelingsperger, C. P., van Berkel, T. J. C., Sluimer, J. C., Temmerman, L., & Biessen, E. A. L. (2019). Low human and murine Mcl-1 expression leads to a pro-apoptotic plaque phenotype enriched in giant-cells. Scientific Reports, 9, [14547].
    • Oliveira da Rocha, G. H., Loiola, R. A., Pantaleao, L. D. N., Reutelingsperger, C., Solito, E., & Poliselli Farsky, S. H. (2019). Control of expression and activity of peroxisome proliferated‐activated receptor γ by Annexin A1 on microglia during efferocytosis. Cell Biochemistry and Function, 37(7), 560-568.
    • Barbosa, C. M., Fock, R. A., Hastreiter, A. A., Reutelingsperger, C., Perretti, M., Paredes-Gamero, E. J., & Farsky, S. H. P. (2019). Extracellular annexin-A1 promotes myeloid/granulocytic differentiation of hematopoietic stem/progenitor cells via the Ca2+/MAPK signalling transduction pathway. Cell death discovery, 5, [135].
    • Van Kuijk, K., Theelen, T., Demandt, J., Marsch, E., Gijbels, M., Jin, H., Reutelingsperger, C., Cosemans, J., Schurgers, L., Carmeliet, P., Biessen, E., Daemen, M., & Sluimer, J. (2019). MYELOID PHD2 KNOCKDOWN DRIVES MACROPHAGE APOPTOSIS AND PARACRINE FIBROBLAST/SMOOTH MUSCLE CELL COLLAGEN SECRETION LEADING TO ATHEROSCLEROTIC PLAQUE FIBROSIS. Atherosclerosis, 287, E10-E10.
    • Everts-Graber, J., Martin, K. R., Thieblemont, N., Mocek, J., Roccabianca, A., Chafey, P., Le Gall, M., Tacnet-Delorme, P., Reutelingsperger, C. P., Naccache, J-M., Bonnotte, B., Karras, A., Puechal, X., Guillevin, L., Terrier, B., Frachet, P., Perretti, M., Mouthon, L., & Witko-Sarsat, V. (2019). Proteomic analysis of neutrophils in ANCA-associated vasculitis reveals a dysregulation in proteinase 3-associated proteins such as annexin-A1 involved in apoptotic cell clearance. Kidney International, 96(2), 397-408.
    • Zhou, Q., Zeng, Y., Xiong, Q., Zhong, S., Li, P., Ran, H., Yin, Y., Reutelingsperger, C., Prinze, F. W., & Ling, Z. (2019). Construction of CNA35 Collagen-Targeted Phase-Changeable Nanoagents for Low-Intensity Focused Ultrasound-Triggered Ultrasound Molecular Imaging of Myocardial Fibrosis in Rabbits. ACS Applied Materials & Interfaces, 11(26), 23006-23017.
    • Petsophonsakul, P., Furmanik, M., Forsythe, R., Dweck, M., Schurink, G. W., Natour, E., Reutelingsperger, C., Jacobs, M., Mees, B., & Schurgers, L. (2019). Role of Vascular Smooth Muscle Cell Phenotypic Switching and Calcification in Aortic Aneurysm Formation Involvement of Vitamin K-Dependent Processes. Arteriosclerosis Thrombosis and Vascular Biology, 39(7), 1351-1368.
    • Ferraro, B., Leoni, G., Hinkel, R., Ormanns, S., Paulin, N., Ortega-Gomez, A., Viola, J. R., de Jong, R., Bongiovanni, D., Bozoglu, T., Maas, S. L., D'Amico, M., Kessler, T., Zeller, T., Hristov, M., Reutelingsperger, C., Sager, H. B., Doering, Y., Nahrendorf, M., ... Soehnlein, O. (2019). Pro-Angiogenic Macrophage Phenotype to Promote Myocardial Repair. Journal of the American College of Cardiology, 73(23), 2990-3002.
    • Purvis, G. S. D., Collino, M., Loiola, R. A., Baragetti, A., Chiazza, F., Brovelli, M., Sheikh, M. H., Collotta, D., Cento, A., Mastrocola, R., Aragno, M., Cutrin, J. C., Reutelingsperger, C., Grigore, L., Catapano, A. L., Yaqoob, M. M., Norata, G. D., Solito, E., & Thiemermann, C. (2019). Identification of AnnexinA1 as an Endogenous Regulator of RhoA, and Its Role in the Pathophysiology and Experimental Therapy of Type-2 Diabetes. Frontiers in Immunology, 10, [571].
    • Gussenhoven, R., Klein, L., Ophelders, D. R. M. G., Habets, D. H. J., Giebel, B., Kramer, B. W., Schurgers, L. J., Reutelingsperger, C. P. M., & Wolfs, T. G. A. M. (2019). Annexin A1 as Neuroprotective Determinant for Blood-Brain Barrier Integrity in Neonatal Hypoxic-Ischemic Encephalopathy. Journal of Clinical Medicine, 8(2), [137].