Hans Ippel

Technical staff

Hans Ippel studied Chemistry at Leiden University and received his PhD degree (1992) in Chemistry at Leiden University as well. He has worked as post-doc researcher in several job positions at Nijmegen University, Umeå University (Sweden), Wageningen University, and Utrecht University, and was also shortly employed as bioinformatician at the biotech company Catchmabs in Wageningen. In 2011, he joined the Biochemistry group of Tilman Hackeng, working as senior researcher doing structural NMR spectroscopy of peptides and proteins. He is currently employed as supervisor of the NMR facility supporting various academic researchers at CARIM and Maastricht UMC+. In addition, he is responsible for assisting external NMR users and doing contract work to third parties. Besides having expertise in structural biophysics and bioinformatics, Hans is an avid collector of Natural Art and all kinds of science stuff.

Research topics of interest are blood coagulation factors and potent inhibitory chemokines and peptides, e.g. derived from ticks. Here, NMR spectroscopy is used to determine 3D structures of native protein and the protein-target complex, in order to come up with new ways of drug targeting. Another research topic is on the structural biology of galectins (such as Gal1, Gal3 and Gal8) that are important proteins involved in cancer development. It is a collaboration project carried out between CARIM and the groups of Prof. Kevin Mayo (Minnesota University) and Prof Christian Weber (LUM München). NMR is also applied for metabolomic profiling of patients blood and urine samples, as well as being used as a structural validation tool towards the design of smart biopolymers.  


Department of Biochemistry
Universiteitsingel 50, 6229 ER Maastricht
PO Box 616, 6200 MD Maastricht
Room number: H4.301
T: +31(0)43-388 24 92

  • 2023
    • Chai, J., Wu, J., Li, J., Liao, H., Lu, W., Guo, R., Shao, Z., Jmel, M. A., Martins, L. A., Hackeng, T., Ippel, H., Dijkgraaf, I., Kotsyfakis, M., & Xu, X. (2023). Novel Amphibian Bowman-Birk-Like Inhibitor with Antioxidant and Anticoagulant Effects Ameliorates Pancreatitis Symptoms in Mice. Journal of Medicinal Chemistry, 66(17), 11869-11880. https://doi.org/10.1021/acs.jmedchem.3c00475
    • Wang, R., Damanik, F., Kuhnt, T., Jaminon, A., Hafeez, S., Liu, H., Ippel, H., Dijkstra, P. J., Bouvy, N., Schurgers, L., ten Cate, A. T., Dias, A., Moroni, L., & Baker, M. B. (2023). Biodegradable Poly(ester) Urethane Acrylate Resins for Digital Light Processing: From Polymer Synthesis to 3D Printed Tissue Engineering Constructs. Advanced Healthcare Materials, 12(17). https://doi.org/10.1002/adhm.202202648
  • 2022
    • Brandhofer, M., Hoffmann, A., Blanchet, X., Siminkovitch, E., Rohlfing, A. K., El Bounkari, O., Nestele, J. A., Bild, A., Kontos, C., Hille, K., Rohde, V., Froehlich, A., Golemi, J., Gokce, O., Krammer, C., Scheiermann, P., Tsilimparis, N., Sachs, N., Kempf, W. E., ... Bernhagen, J. (2022). Heterocomplexes between the atypical chemokine MIF and the CXC-motif chemokine CXCL4L1 regulate inflammation and thrombus formation. Cellular and Molecular Life Sciences, 79(10), Article 512. https://doi.org/10.1007/s00018-022-04539-0
    • Leberzammer, J., Agten, S. M., Blanchet, X., Duan, R., Ippel, H., Megens, R. T. A., Schulz, C., Aslani, M., Duchene, J., Döring, Y., Jooss, N. J., Zhang, P., Brandl, R., Stark, K., Siess, W., Jurk, K., Heemskerk, J. W. M., Hackeng, T. M., Mayo, K. H., ... von Hundelshausen, P. (2022). Targeting platelet-derived CXCL12 impedes arterial thrombosis. Blood, 139(17), 2691-2705. https://doi.org/10.1182/blood.2020010140
    • Poelman, H., Ippel, H., Gurkan, B., Boelens, R., Vriend, G., van 't Veer, C., Lutgens, E., & Nicolaes, G. A. F. (2022). Structural anomalies in a published NMR-derived structure of IRAK-M. Journal of Molecular Graphics & Modelling, 111, Article 108061. https://doi.org/10.1016/j.jmgm.2021.108061
  • 2021
    • Bervoets, L., Ippel, J. H., Smolinska, A., van Best, N., Savelkoul, P. H. M., Mommers, M. A. H., & Penders, J. (2021). Practical and Robust NMR-Based Metabolic Phenotyping of Gut Health in Early Life. Journal of Proteome Research, 20(11), 5079-5087. https://doi.org/10.1021/acs.jproteome.1c00617
    • Denisov, S. S., Ippel, J. H., Castoldi, E., Mans, B. J., Hackeng, T. M., & Dijkgraaf, I. (2021). Molecular basis of anticoagulant and anticomplement activity of the tick salivary protein Salp14 and its homologs. Journal of Biological Chemistry, 297(1), Article 100865. https://doi.org/10.1016/j.jbc.2021.100865
    • Miller, M. C., Zheng, Y., Suylen, D., Ippel, H., Canada, F. J., Berbis, M. A., Jimenez-Barbero, J., Tai, G., Gabius, H-J., & Mayo, K. H. (2021). Targeting the CRD F-face of Human Galectin-3 and Allosterically Modulating Glycan Binding by Angiostatic PTX008 and a Structurally Optimized Derivative. Chemmedchem, 16(4), 713-723. https://doi.org/10.1002/cmdc.202000742
  • 2020
    • Denisov, S. S., Ramirez-Escudero, M., Heinzmann, A. C. A., Ippel, J. H., Dawson, P. E., Koenen, R. R., Hackeng, T. M., Janssen, B. J. C., & Dijkgraaf, I. (2020). Structural characterization of anti-CCL5 activity of the tick salivary protein evasin-4. Journal of Biological Chemistry, 295(42), 14367-14378. https://doi.org/10.1074/jbc.RA120.013891
    • Weterings, J., Rijcken, C. J. F., Veldhuis, H., Meulemans, T., Hadavi, D., Timmers, M., Honing, M., Ippel, H., & Liskamp, R. M. J. (2020). TMTHSI, a superior 7-membered ring alkyne containing reagent for strain-promoted azide-alkyne cycloaddition reactions. Chemical Science, 11(33), 9011-9016. https://doi.org/10.1039/d0sc03477k
    • Miller, M. C., Nesmelova, I. V., Daragan, V. A., Ippel, H., Michalak, M., Dregni, A., Kaltner, H., Kopitz, J., Gabius, H. J., & Mayo, K. H. (2020). Pro4 prolyl peptide bond isomerization in human galectin-7 modulates the monomer-dimer equilibrum to affect function. Biochemical Journal, 477(17), 3147-3165. https://doi.org/10.1042/BCJ20200499
    • Eckardt, V., Miller, M. C., Blanchet, X., Duan, R., Leberzammer, J., Duchene, J., Soehnlein, O., Megens, R. T. A., Ludwig, A-K., Dregni, A., Faussner, A., Wichapong, K., Ippel, H., Dijkgraaf, I., Kaltner, H., Doering, Y., Bidzhekov, K., Hackeng, T. M., Weber, C., ... Mayo, K. H. (2020). Chemokines and galectins form heterodimers to modulate inflammation. Embo Reports, 21(4), Article 47852. https://doi.org/10.15252/embr.201947852
    • Denisov, S. S., Heinzmann, A. C. A., Vajen, T., Vries, M. H. M., Megens, R. T. A., Suylen, D., Koenen, R. R., Post, M. J., Ippel, J. H., Hackeng, T. M., & Dijkgraaf, I. (2020). Tick Saliva Protein Evasin-3 Allows for Visualization of Inflammation in Arteries through Interactions with CXC-Type Chemokines Deposited on Activated Endothelium. Bioconjugate Chemistry, 31(3), 948-955. https://doi.org/10.1021/acs.bioconjchem.0c00095
  • 2019
    • Zhang, H., Ippel, H., Miller, M. C., Wong, T. J., Griffioen, A. W., Mayo, K. H., & Pieters, R. J. (2019). Hybrid ligands with calixarene and thiodigalactoside groups: galectin binding and cytotoxicity. Organic Chemistry Frontiers, 6(16), 2981-2990. https://doi.org/10.1039/c9qo00810a
    • Denisov, S. S., Ippel, J. H., Heinzmann, A. C. A., Koenen, R. R., Ortega-Gomez, M., Soehnlein, O., Hackeng, T. M., & Dijkgraaf, I. (2019). Tick saliva protein Evasin-3 modulates chemotaxis by disrupting CXCL8 interactions with glycosaminoglycans and CXCR2. Journal of Biological Chemistry, 294(33), 12370-12379. https://doi.org/10.1074/jbc.RA119.008902
    • Streefkerk, D. E., Schmidt, M., Ippel, J. H., Hackeng, T. M., Nuijens, T., Timmerman, P., & van Maarseveen, J. H. (2019). Synthesis of Constrained Tetracyclic Peptides by Consecutive CEPS, CLIPS, and Oxime Ligation. Organic Letters, 21(7), 2095-2100. https://doi.org/10.1021/acs.orglett.9b00378
    • Denisov, S. S., Ippel, J. H., Mans, B. J., Dijkgraaf, I., & Hackeng, T. M. (2019). SecScan: a general approach for mapping disulfide bonds in synthetic and recombinant peptides and proteins. Chemical Communications, 55(10), 1374-1377. https://doi.org/10.1039/c8cc08777f
  • 2018
    • Balthasar, A. J. R., Bydlon, T. M., Ippel, H., van der Voort, M., Hendriks, B. H. W., Lucassen, G. W., van Geffen, G-J., van Kleef, M., van Dijk, P., & Lataster, A. (2018). Optical signature of nerve tissue-Exploratory ex vivo study comparing optical, histological, and molecular characteristics of different adipose and nerve tissues. Lasers in Surgery and Medicine, 50(9), 948-960. https://doi.org/10.1002/lsm.22938
    • Richelle, G. J. J., Schmidt, M., Ippel, H., Hackeng, T. M., van Maarseveen, J. H., Nuijens, T., & Timmerman, P. (2018). A One-Pot "Triple-C" Multicyclization Methodology for the Synthesis of Highly Constrained Isomerically Pure Tetracyclic Peptides. Chembiochem, 19(18), 1934-1938. https://doi.org/10.1002/cbic.201800346
  • 2017
    • Schmidt, M., Toplak, A., Quaedflieg, P. J. L. M., Ippel, H., Richelle, G. J. J., Hackeng, T. M., van Maarseveen, J. H., & Nuijens, T. (2017). Omniligase-1: A Powerful Tool for Peptide Head-to-Tail Cyclization. Advanced Synthesis & Catalysis, 359(12), 2050-2055. https://doi.org/10.1002/adsc.201700314
    • von Hundelshausen, P., Agten, S. M., Eckardt, V., Blanchet, X., Schmitt, M. M., Ippel, H., Neideck, C., Bidzhekov, K., Leberzammer, J., Wichapong, K., Faussner, A., Drechsler, M., Grommes, J., van Geffen, J. P., Li, H., Ortega-Gomez, A., Megens, R. T. A., Naumann, R., Dijkgraaf, I., ... Weber, C. (2017). Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation. Science Translational Medicine, 9(384), Article eaah6650. https://doi.org/10.1126/scitranslmed.aah6650
  • 2016
    • Agten, S. M., Koenen, R. R., Ippel, H., Eckardt, V., von Hundelshausen, P., Mayo, K. H., Weber, C., & Hackeng, T. M. (2016). Probing Functional Heteromeric Chemokine Protein-Protein Interactions through Conformation-Assisted Oxime Ligation. Angewandte Chemie-International Edition, 55(48), 14963-14966. https://doi.org/10.1002/anie.201607036
    • Ippel, H., Miller, M. C., Vertesy, S., Zheng, Y., Javier Canada, F., Suylen, D., Umemoto, K., Romano, C., Hackeng, T., Tai, G., Leffler, H., Kopitz, J., Andre, S., Kuebler, D., Jimenez-Barbero, J., Oscarson, S., Gabius, H-J., & Mayo, K. H. (2016). Intra- and intermolecular interactions of human galectin-3: assessment by full-assignment-based NMR. Glycobiology, 26(8), 888-903. https://doi.org/10.1093/glycob/cww021
    • Miller, M. C., Ippel, H., Suylen, D., Klyosov, A. A., Traber, P. G., Hackeng, T., & Mayo, K. H. (2016). Binding of polysaccharides to human galectin-3 at a noncanonical site in its carbohydrate recognition domain. Glycobiology, 26(1), 88-99. https://doi.org/10.1093/glycob/cwv073
  • 2015
    • Alard, J-E., Ortega-Gomez, A., Wichapong, K., Bongiovanni, D., Horckmans, M., Megens, R. T. A., Leoni, G., Ferraro, B., Rossaint, J., Paulin, N., Ng, J., Ippel, H., Suylen, D., Hinkel, R., Blanchet, X., Gaillard, F., D'Amico, M., von Hundelshausen, P., Zarbock, A., ... Soehnlein, O. (2015). Recruitment of classical monocytes can be inhibited by disturbing heteromers of neutrophil HNP1 and platelet CCL5. Science Translational Medicine, 7(317), Article 317ra196. https://doi.org/10.1126/scitranslmed.aad5330
    • Hu, Q., van Gaal, E. V. B., Brundel, P., Ippel, H., Hackeng, T., Rijcken, C. J. F., Storm, G., Hennink, W. E., & Prakash, J. (2015). A novel approach for the intravenous delivery of leuprolide using core-cross-linked polymeric micelles. Journal of Controlled Release, 205, 98-108. https://doi.org/10.1016/j.jconrel.2014.12.023
    • Ippel, H., Miller, M. C., Berbis, M. A., Suylen, D., Andre, S., Hackeng, T. M., Canada, F. J., Weber, C., Gabius, H-J., Jimenez-Barbero, J., & Mayo, K. H. (2015). H-1, C-13, and N-15 backbone and side-chain chemical shift assignments for the 36 proline-containing, full length 29 kDa human chimera-type galectin-3. Biomolecular Nmr Assignments, 9(1), 59-63. https://doi.org/10.1007/s12104-014-9545-3
  • 2014
    • Karagoz, G. E., Duarte, A. M. S., Akoury, E., Ippel, H., Biernat, J., Luengo, T. M., Radli, M., Didenko, T., Nordhues, B. A., Veprintsev, D. B., Dickey, C. A., Mandelkow, E., Zweckstetter, M., Boelens, R., Madl, T., & Rudiger, S. G. D. (2014). Hsp90-Tau Complex Reveals Molecular Basis for Specificity in Chaperone Action. Cell, 156(5), 963-974. https://doi.org/10.1016/j.cell.2014.01.037
    • Berbis, M. A., Andre, S., Canada, F. J., Pipkorn, R., Ippel, H., Mayo, K. H., Kuebler, D., Gabius, H-J., & Jimenez-Barbero, J. (2014). Peptides derived from human galectin-3 N-terminal tail interact with its carbohydrate recognition domain in a phosphorylation-dependent manner. Biochemical and Biophysical Research Communications, 443(1), 126-131. https://doi.org/10.1016/j.bbrc.2013.11.063
  • 2013
    • Agten, S. M., Suylen, D., Ippel, H., Kokozidou, M., Tans, G., van de Vijver, P., Koenen, R. R., & Hackeng, T. M. (2013). Chemoselective Oxime Reactions in Proteins and Peptides by Using an Optimized Oxime Strategy: The Demise of Levulinic Acid. Chembiochem, 14(18), 2431-2434. https://doi.org/10.1002/cbic.201300598
    • van der Kolk, J. H., Boelens, R., Halkes, S. B. A., Wijnberg, I. D., de Sain-van der Velden, M. G. M., & Ippel, J. H. (2013). Some notes on fatal acquired multiple acyl-CoA dehydrogenase deficiency (MADD) in a two-year-old warmblood stallion and European tar spot (Rhytisma acerinum). Veterinary Quarterly, 33(1), 51-55. https://doi.org/10.1080/01652176.2012.758904
    • Kumar, N., Ippel, H., Weber, C., Hackeng, T., & Mayo, K. H. (2013). Protein lysine-N zeta alkylation and O-phosphorylation mediated by DTT-generated reactive oxygen species. Protein Science, 22(3), 327-346. https://doi.org/10.1002/pro.2214