Henri Spronk

Associate professor

Initially trained in Biochemistry and Molecular Biology at Nijmegen University, Dr Henri Spronk obtained a PhD in Biochemistry on the effects of impaired vitamin K-dependent protein carboxylation.

The focus of his current work is on the bidirectional relation between coagulation and inflammation, as well as on the applicability of functional plasma assays such as thrombin generation, for atherothrombotic risk prediction. He has a special interest in the role of active coagulation factors, mainly thrombin, in activation of protein-activated receptors (PARs) which can regulate the balance between vascular protective and disruptive. Overall, knowledge on the non-haemostatic functions of thrombin and factor Xa enables pharmacological interference in cellular processes contributing to the the development and progression of atherosclerosis or atrial fibrillation.

Department of Biochemistry
Department of Internal Medicine
Universiteitssingel 50, 6229 ER Maastricht
PO Box 616, 6200 MD Maastricht
Room number: 4.326
T: +31(0)43 388 42 61

  • 2015
    • Spronk, H. M. H., Cannegieter, S., Morange, P., Hackeng, T., Huisman, M., Nagler, M., Posthuma, J., Ninivaggi, M., Zwaveling, S., van der Hulle, T., Scheres, L. J. J., van Mens, T. E., & Mackman, N. (2015). Theme 2: Epidemiology, Biomarkers, and Imaging of Venous Thromboembolism (and postthrombotic syndrome). Thrombosis Research, 136, S8-S12. https://doi.org/10.1016/j.thromres.2015.07.035
    • Loeffen, R., Winckers, K., Ford, I., Jukema, J. W., Robertson, M., Stott, D. J., Spronk, H. M., ten Cate, H., & Lowe, G. D. (2015). Associations Between Thrombin Generation and the Risk of Cardiovascular Disease in Elderly Patients: Results From the PROSPER Study. Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 70(8), 982-988. https://doi.org/10.1093/gerona/glu228
    • Loeffen, R., Godschalk, T. C., van Oerle, R., Spronk, H. M. H., Hackeng, C. M., ten Berg, J. M., & ten Cate, H. (2015). The hypercoagulable profile of patients with stent thrombosis. Heart, 101(14), 1126-1132. https://doi.org/10.1136/heartjnl-2014-306685
    • Spronk, H. M. H., Braunschweig, T., Rossaint, R., Wuest, D. C., van Oerle, R., Lauritzen, B., Tolba, R., & Grottke, O. (2015). Recombinant Factor VIIa Reduces Bleeding after Blunt Liver Injury in a Pig Model of Dilutional Coagulopathy under Severe Hypothermia. PLOS ONE, 10(6), [e0113979]. https://doi.org/10.1371/journal.pone.0113979
    • Hermanns, M. I., Grossmann, V., Spronk, H. M. H., Schulz, A., Juenger, C., Laubert-Reh, D., Mazur, J., Gori, T., Zeller, T., Pfeiffer, N., Beutel, M., Blankenberg, S., Muenzel, T., Lackner, K. J., Hoek - ten Cate, A. J., ten Cate, H., & Wild, P. S. (2015). Distribution, genetic and cardiovascular determinants of FVIII:c - Data from the population-based Gutenberg Health Study. International Journal of Cardiology, 187, 166-174. https://doi.org/10.1016/j.ijcard.2015.03.330
    • Geenen, I. L. A., Molin, D. G. M., van den Akker, N. M. S., Jeukens, F., Spronk, H. M., Schurink, G. W. H., & Post, M. J. (2015). Endothelial cells (ECs) for vascular tissue engineering: venous ECs are less thrombogenic than arterial ECs. Journal of Tissue Engineering and Regenerative Medicine, 9(5), 564-576. https://doi.org/10.1002/term.1642
    • Posthuma, J. J., van der Meijden, P. E. J., ten Cate, H., & Spronk, H. M. H. (2015). Short- and Long-term exercise induced alterations in haemostasis: a review of the literature. Blood Reviews, 29(3), 171-178. https://doi.org/10.1016/j.blre.2014.10.005
  • 2014
    • Loeffen, R., van Oerle, R., de Groot, P. G., Waltenberger, J., Crijns, H. J., Spronk, H. M. H., & ten Cate, H. (2014). Increased factor XIa levels in patients with a first acute myocardial infarction: The introduction of a new thrombin generation based factor XIa assay. Thrombosis Research, 134(6), 1328-1334. https://doi.org/10.1016/j.thromres.2014.09.015
    • Schurgers, L. J., & Spronk, H. M. H. (2014). Differential cellular effects of old and new oral anticoagulants: consequences to the genesis and progression of atherosclerosis. Thrombosis and Haemostasis, 112(5), 909-917. https://doi.org/10.1160/TH14-03-0268
    • Kleinegris, M. C., Bos, M. H., Roest, M., Henskens, Y., Ten Cate-Hoek, A., Van Deursen, C., Spronk, H. M., Reitsma, P. H., De Groot, P. G., ten Cate, H., & Koek, G. (2014). Cirrhosis patients have a coagulopathy that is associated with decreased clot formation capacity. Journal of Thrombosis and Haemostasis, 12(10), 1647-1657. https://doi.org/10.1111/jth.12706
    • Posthuma, J. J., Loeffen, R., van Oerle, R., Henskens, Y. M. C., ten Cate, H., Spronk, H. M. H., & van der Meijden, P. E. J. (2014). Long-term strenuous exercise induces a hypercoagulable state through contact activation. Thrombosis and Haemostasis, 111(6), 1197-1199. https://doi.org/10.1160/TH13-11-0963
    • Spronk, H. M. H., ten Cate, H., & van der Meijden, P. E. J. (2014). Differential roles of Tissue Factor and Phosphatidylserine in activation of coagulation. Thrombosis Research, 133, S54-S56. https://doi.org/10.1016/j.thromres.2014.03.022
    • Geenen, I. L., Verbruggen, S., Molin, D. G., Spronk, H. M., Maessen, J. G., Meesters, B., Schurink, G. W., & Post, M. J. (2014). Phenotypic Fitness of Primary Endothelial Cells Cultured from Patients with High Cardiovascular Risk or Chronic Kidney Disease for Vascular Tissue Engineering. Tissue Engineering, 20(5-6), 1049-1059. https://doi.org/10.1089/ten.tea.2013.0163
    • Spronk, H. M. H., de Jong, A. M., Crijns, H. J., Schotten, U., Van Gelder, I. C., & ten Cate, H. (2014). Pleiotropic effects of factor Xa and thrombin: what to expect from novel anticoagulants. Cardiovascular Research, 101(3), 344-351. https://doi.org/10.1093/cvr/cvt343
    • Kalz, J., ten Cate, H., & Spronk, H. M. H. (2014). Thrombin generation and atherosclerosis. Journal of Thrombosis and Thrombolysis, 37(1), 45-55. https://doi.org/10.1007/s11239-013-1026-5
    • Cornelis, T., Broers, N. J. H., Titulaer, D. C. L. M., Henskens, Y. M., van Oerle, R., van der Sande, F. M., Spronk, H. M., Vink, H., Leunissen, K. M., ten Cate, H., & Kooman, J. P. (2014). Effects of Ultrapure Hemodialysis and Low Molecular Weight Heparin on the Endothelial Surface Layer. Blood Purification, 38(3-4), 203-210. https://doi.org/10.1159/000369055
    • Grottke, O., van Ryn, J., Spronk, H. M. H., & Rossaint, R. (2014). Prothrombin complex concentrates and a specific antidote to dabigatran are effective ex-vivo in reversing the effects of dabigatran in an anticoagulation/liver trauma experimental model. Critical Care, 18(1), [R27]. https://doi.org/10.1186/cc13717
  • 2013
    • Spronk, H. M. H., Borissoff, J. I., & ten Cate, H. (2013). New Insights into Modulation of Thrombin Formation. Current Atherosclerosis Reports, 15(11), [363]. https://doi.org/10.1007/s11883-013-0363-3
    • Konings, J., Govers-Riemslag, J. W. P., Spronk, H. M. H., Waltenberger, J. L., & ten Cate, H. (2013). Activation of the contact system in patients with a first acute myocardial infarction. Thrombosis Research, 132(1), 138-142. https://doi.org/10.1016/j.thromres.2013.05.016
    • Smid, M., Dielis, A. W. J. H., Spronk, H. M. H., Rumley, A., van Oerle, R., Woodward, M., ten Cate, H., & Lowe, G. (2013). Thrombin Generation in the Glasgow Myocardial Infarction Study. PLOS ONE, 8(6), [e66977]. https://doi.org/10.1371/journal.pone.0066977
    • Theuwissen, E., Teunissen, K. J., Spronk, H. M. H., Hamulyak, K., Ten Cate, H., Shearer, M. J., Vermeer, C., & Schurgers, L. J. (2013). Effect of low-dose supplements of menaquinone-7 (vitamin K-2) on the stability of oral anticoagulant treatment: dose-response relationship in healthy volunteers. Journal of Thrombosis and Haemostasis, 11(6), 1085-1092. https://doi.org/10.1111/jth.12203
    • van der Meijden, P. E. J., Bouman, A. C., Feijge, M. A. H., van Oerle, R., Spronk, H. M. H., Hamulyak, K., ten Cate-Hoek, A. J., ten Cate, H., & Heemskerk, J. W. M. (2013). Platelet Dysfunction in Thrombosis Patients Treated with Vitamin K Antagonists and Recurrent Bleeding. PLOS ONE, 8(5). https://doi.org/10.1371/journal.pone.0064112
    • Strak, M., Hoek, G., Steenhof, M., Kilinc, E., Godri, K. J., Gosens, I., Mudway, I. S., van Oerle, R., Spronk, H. M. H., Cassee, F. R., Kelly, F. J., Harrison, R. M., Brunekreef, B., Lebret, E., & Janssen, N. A. H. (2013). Components of ambient air pollution affect thrombin generation in healthy humans: the RAPTES project. Occupational and Environmental Medicine, 70(5), 332-340. https://doi.org/10.1136/oemed-2012-100992
    • Strak, M., Hoek, G., Godri, K. J., Gosens, I., Mudway, I. S., van Oerle, R., Spronk, H. M. H., Cassee, F. R., Lebret, E., Kelly, F. J., Harrison, R. M., Brunekreef, B., Steenhof, M., & Janssen, N. A. H. (2013). Composition of PM Affects Acute Vascular Inflammatory and Coagulative Markers - The RAPTES Project. PLOS ONE, 8(3), [e58944]. https://doi.org/10.1371/journal.pone.0058944
    • Borissoff, J. I., Otten, J. J. T., Heeneman, S., Leenders, P., van Oerle, R., Soehnlein, O., Loubele, S. T. B. G., Hamulyak, K., Hackeng, T. M., Daemen, M. J. A. P., Degen, J. L., Weiler, H., Esmon, C. T., van Ryn, J., Biessen, E. A. L., Spronk, H. M. H., & ten Cate, H. (2013). Genetic and Pharmacological Modifications of Thrombin Formation in Apolipoprotein E-deficient Mice Determine Atherosclerosis Severity and Atherothrombosis Onset in a Neutrophil-Dependent Manner. PLOS ONE, 8(2), [e55784]. https://doi.org/10.1371/journal.pone.0055784
    • Lance, M. D., Henskens, Y., Nelemans, P., Theunissen, M. H. S., Van Oerle, R., Spronk, H. M., & Marcus, M. A. E. (2013). Do blood collection methods influence whole-blood platelet function analysis?Platelets, 24(4), 275-281. https://doi.org/10.3109/09537104.2012.689038
  • 2012
    • Borissoff, J. I., Joosen, I. A., Versteylen, M. O., Spronk, H. M., ten Cate, H., & Hofstra, L. (2012). Accelerated In Vivo Thrombin Formation Independently Predicts the Presence and Severity of CT Angiographic Coronary Atherosclerosis. JACC-Cardiovascular Imaging, 5(12), 1201-1210. https://doi.org/10.1016/j.jcmg.2012.01.023
    • Dargaud, Y., Wolberg, A. S., Luddington, R., Regnault, V., Spronk, H., Baglin, T., Lecompte, T., Ten Cate, H., & Negrier, C. (2012). Evaluation of a standardized protocol for thrombin generation measurement using the calibrated automated thrombogram: An international multicentre study. Thrombosis Research, 130(6), 929-934. https://doi.org/10.1016/j.thromres.2012.07.017
    • Loeffen, R., Kleinegris, M. -C. F., Loubele, S. T. B. G., Pluijmen, P. H. M., Fens, D., van Oerle, R., ten Cate, H., & Spronk, H. M. H. (2012). Preanalytic variables of thrombin generation: towards a standard procedure and validation of the method. Journal of Thrombosis and Haemostasis, 10(12), 2544-2554. https://doi.org/10.1111/jth.12012
    • Geenen, I. L., Post, M. J., Molin, D. G. M., Schurink, G. W. H., Maessen, J. G., van Oerle, R., ten Cate, H., & Spronk, H. M. H. (2012). Coagulation on endothelial cells: the underexposed part of Virchow's Triad. Thrombosis and Haemostasis, 108(5), 863-871. https://doi.org/10.1160/TH12-04-0269
    • Talens, S., Malfliet, J. J. M. C., Rudez, G., Spronk, H. M. H., Janssen, N. A. H., Meijer, P., Kluft, C., de Maat, M. P. M., & Rijken, D. C. (2012). Biological variation in tPA-induced plasma clot lysis time. Thrombosis and Haemostasis, 108(4), 640-646. https://doi.org/10.1160/TH12-02-0124
    • Lance, M. D., Kuiper, G. J. A. J. M., Sloep, M., Spronk, H. M. H., van Oerle, R., ten Cate, H., Marcus, M. A. E., & Henskens, Y. M. C. (2012). The effects of pneumatic tube system transport on ROTEM analysis and contact activation assessed by thrombin generation test. Thrombosis Research, 130(3), E147-E150. https://doi.org/10.1016/j.thromres.2012.05.002
    • van der Meijden, P. E. J., van Schilfgaarde, M., van Oerle, R., Renne, T., ten Cate, H., & Spronk, H. M. H. (2012). Platelet- and erythrocyte-derived microparticles trigger thrombin generation via factor XIIa. Journal of Thrombosis and Haemostasis, 10(7), 1355-1362. https://doi.org/10.1111/j.1538-7836.2012.04758.x
    • Loeffen, R., Spronk, H. M. H., & ten Cate, H. (2012). The impact of blood coagulability on atherosclerosis and cardiovascular disease. Journal of Thrombosis and Haemostasis, 10(7), 1207-1216. https://doi.org/10.1111/j.1538-7836.2012.04782.x
    • Beijers, H. J. B. H., Ferreira, I., Spronk, H. M. H., Bravenboer, B., Dekker, J. M., Nijpels, G., ten Cate, H., & Stehouwer, C. D. A. (2012). Impaired glucose metabolism and type 2 diabetes are associated with hypercoagulability: potential role of central adiposity and low-grade inflammation - The Hoorn Study. Thrombosis Research, 129(5), 557-562. https://doi.org/10.1016/j.thromres.2011.07.033
    • Trappenburg, M. C., van Schilfgaarde, M., Frerichs, F. C. P., Spronk, H. M. H., ten Cate, H., de Fijter, C. W. H., Terpstra, W. E., & Leyte, A. (2012). Chronic renal failure is accompanied by endothelial activation and a large increase in microparticle numbers with reduced procoagulant capacity. Nephrology Dialysis Transplantation, 27(4), 1446-1453. https://doi.org/10.1093/ndt/gfr474
    • Lecut, C., Faccinetto, C., Delierneux, C., Van Oerle, R., Spronk, H. M. H., Evans, R. J., El Benna, J., Bours, V., & Oury, C. (2012). ATP-gated P2X1 ion channels protect against endotoxemia by dampening neutrophil activation. Journal of Thrombosis and Haemostasis, 10(3), 453-465. https://doi.org/10.1111/j.1538-7836.2011.04606.x
  • 2011
    • Mahmoodi, B. K., Mulder, A. B., Waanders, F., Spronk, H. M. H., Mulder, E. R., Slagman, M. C. J., Vogt, L., Navis, G., Ten Cate, H., Kluin-Nelemans, H. C., & Laverman, G. D. (2011). The impact of antiproteinuric therapy on the prothrombotic state in patients with overt proteinuria. Journal of Thrombosis and Haemostasis, 9(12), 2416-2423. https://doi.org/10.1111/j.1538-7836.2011.04525.x
    • Winckers, K., Siegerink, B., Duckers, C., Maurissen, L. F., Tans, G., Castoldi, E., Spronk, H. M. H., Ten Cate, H., Algra, A., Hackeng, T. M., & Rosendaal, F. R. (2011). Increased tissue factor pathway inhibitor activity is associated with myocardial infarction in young women: results from the RATIO study. Journal of Thrombosis and Haemostasis, 9(11), 2243-2250. https://doi.org/10.1111/j.1538-7836.2011.04497.x
    • Honickel, M., Rieg, A. D., Rossaint, R., Braunschweig, T., Spronk, H. M. H., ten Cate, H., van Oerle, R., Tolba, R., & Grottke, O. (2011). Prothrombin complex concentrate reduces blood loss and enhances thrombin generation in a pig model with blunt liver injury under severe hypothermia. Thrombosis and Haemostasis, 106(4), 724-733. https://doi.org/10.1160/TH11-02-0101
    • Grottke, O., Braunschweig, T., Spronk, H. M. H., Esch, S., Rieg, A. D., van Oerle, R., ten Cate, H., Fitzner, C., Tolba, R., & Rossaint, R. (2011). Increasing concentrations of prothrombin complex concentrate induce disseminated intravascular coagulation in a pig model of coagulopathy with blunt liver injury. Blood, 118(7), 1943-1951. https://doi.org/10.1182/blood-2011-03-343046
    • Kilinc, E., Van Oerle, R., Borissoff, J. I., Oschatz, C., Gerlofs Nijland, M. E., Janssen, N. A., Cassee, F. R., Sandstrom, T., Renne, T., Ten Cate, H., & Spronk, H. M. H. (2011). Factor XII activation is essential to sustain the procoagulant effects of particulate matter. Journal of Thrombosis and Haemostasis, 9(7), 1359-1367. https://doi.org/10.1111/j.1538-7836.2011.04280.x
    • Borissoff, J. I., Spronk, H. M. H., & ten Cate, H. (2011). MECHANISMS OF DISEASE The Hemostatic System as a Modulator of Atherosclerosis. New England Journal of Medicine, 364(18), 1746-1760. https://doi.org/10.1056/NEJMra1011670
    • Trappenburg, M. C., van Schilfgaarde, M., Bredewold, E. O., van Aalderen, M. C., Spronk, H. M. H., ten Cate, H., Leyte, A., & Terpstra, W. E. (2011). Elevated numbers and altered subsets of procoagulant microparticles in breast cancer patients using endocrine therapy. Thrombosis Research, 127(4), 363-369. https://doi.org/10.1016/j.thromres.2010.12.015
    • Panova-Noeva, M., Marchetti, M., Spronk, H. M., Russo, L., Diani, E., Finazzi, G., Salmoiraghi, S., Rambaldi, A., Barbui, T., Ten Cate, H., & Falanga, A. (2011). Platelet-induced thrombin generation by the calibrated automated thrombogram assay is increased in patients with essential thrombocythemia and polycythemia vera. American Journal of Hematology, 86(4), 337-342. https://doi.org/10.1002/ajh.21974
    • Kilinc, E., Schulz, H., Kuiper, G. J. A. J. M., Spronk, H. M. H., ten Cate, H., Upadhyay, S., Ganguly, K., Stoeger, T., Semmler-Bhenke, M., Takenaka, S., Kreyling, W. G., Pitz, M., Reitmeir, P., Peters, A., Eickelberg, O., & Wichmann, H. E. (2011). The procoagulant effects of fine particulate matter in vivo. Particle and Fibre Toxicology, 8, 3. https://doi.org/10.1186/1743-8977-8-12
    • Smid, M., Dielis, A. W. J. H., Winkens, M. H. M., Spronk, H. M. H., van Oerle, R., Hamulyak, K., Prins, M. H., Rosing, J., Waltenberger, J. L., & ten Cate, H. (2011). Thrombin generation in patients with a first acute myocardial infarction. Journal of Thrombosis and Haemostasis, 9(3), 450-456. https://doi.org/10.1111/j.1538-7836.2010.04162.x
  • 2010
    • Beijers, H. J. B. H., Ferreira, I., Spronk, H. M. H., Bravenboer, B., Dekker, J. M., Nijpels, G., ten Cate, H., & Stehouwer, C. D. A. (2010). Body composition as determinant of thrombin generation in plasma: the Hoorn study.Arteriosclerosis Thrombosis and Vascular Biology, 30(12), 2639-2647. https://doi.org/10.1161/ATVBAHA.110.211946
    • Gerlofs-Nijland, M. E., Totlandsdal, A. I., Kilinc, E., Boere, J. A. F., Fokkens, P. H. B., Leseman, D. L. A. C., Sioutas, C., Schwarze, P. E., Spronk, H. M., Hadoke, P. W. F., Miller, M. R., & Cassee, F. R. (2010). Pulmonary and cardiovascular effects of traffic-related particulate matter: 4-week exposure of rats to roadside and diesel engine exhaust particles. Inhalation Toxicology, 22(14), 1162-1173. https://doi.org/10.3109/08958378.2010.531062
    • Dargaud, Y., Spronk, H. M. H., Leenders, P., Hemker, H. C., & Ten Cate, H. (2010). Monitoring platelet dependent thrombin generation in mice. Thrombosis Research, 126(5), 436-441. https://doi.org/10.1016/j.thromres.2010.08.007