Johan Heemskerk


Prof. Johan Heemskerk graduated in Biology and Chemistry at Radboud University Nijmegen. At the same university, he performed doctoral research on the biosynthesis of plant lipids, with post-doctoral projects at the universities of Hamburg, Helsinki and Cambridge (UK). In 1986, he moved to Maastricht University, with positions at the Departments of Human Biology and Biochemistry. He is a recipient of the BACH-ISTH award for contributions to hemostasis. He participates in the following international consortia: European Platelet Network (EUPLAN); BRIDGE-UK, Horizon 2020 TAPAS and TICARDIO, Interreg EMR PolyValve.

Since 2000, he is a Principal Investigator at CARIM, where he studies signal transduction processes and cell-cell communication in blood cells in relation to hemostasis, thrombosis, atherosclerosis, vascular biology and nutrition. Main topics in his programme are the biochemical, cell-biological and physiological regulation of platelet-dependent coagulation and the importance of this process for thrombus formation, stability and embolism. His research encompasses experimental and intervention studies with blood cells from both human and animal sources, with an emphasis on flow-dependent perfusion models using microfluidics. Within consortia, his laboratory establishes platelet proteomes in mice, healthy subjects and defined patients.

Department of Biochemistry
Universiteitssingel 50, 6229 ER Maastricht
PO Box 616, 6200 MD Maastricht
Room: H4.308

  • 2020
    • van Geffen, J. P., Swieringa, F., van Kuijk, K., Tullemans, B. M. E., Solari, F. A., Peng, B., Clemetson, K. J., Farndale, R. W., Dubois, L. J., Sickmann, A., Zahedi, R. P., Ahrends, R., Biessen, E. A. L., Sluimer, J. C., Heemskerk, J. W. M., & Kuijpers, M. J. E. (2020). Mild hyperlipidemia in mice aggravates platelet responsiveness in thrombus formation and exploration of platelet proteome and lipidome. Scientific Reports, 10(1), [21407].
    • Heinzmann, A. C. A., Karel, M. F. A., Coenen, D. M., Vajen, T., Meulendijks, N. M. M., Nagy, M., Suylen, D. P. L., Cosemans, J. M. E. M., Heemskerk, J. W. M., Hackeng, T. M., & Koenen, R. R. (2020). Complementary roles of platelet alpha(IIb)beta(3) integrin, phosphatidylserine exposure and cytoskeletal rearrangement in the release of extracellular vesicles. Atherosclerosis, 310, 17-25.
    • Veninga, A., De Simone, I., Heemskerk, J. W. M., Ten Cate, H., & van der Meijden, P. E. J. (2020). Clonal hematopoietic mutations linked to platelet traits and the risk of thrombosis or bleeding. Haematologica-the Hematology Journal, 105(8), 2020-2031.
    • Brouns, S. L. N., van Geffen, J. P., Campello, E., Swieringa, F., Spiezia, L., van Oerle, R., Provenzale, I., Verdoold, R., Farndale, R. W., Clemetson, K. J., Spronk, H. M. H., van der Meijden, P. E. J., Cavill, R., Kuijpers, M. J. E., Castoldi, E., Simioni, P., & Heemskerk, J. W. M. (2020). Platelet-primed interactions of coagulation and anticoagulation pathways in flow-dependent thrombus formation. Scientific Reports, 10(1), 11910. [11910].
    • Nagy, M., Perrella, G., Dalby, A., Becerra, M. F., Quintanilla, L. G., Pike, J. A., Morgan, N., Gardiner, E. E., Heemskerk, J. W. M., Azocar, L., Miquel, J. F., Mezzano, D., & Watson, S. P. (2020). Flow studies on human GPVI-deficient blood under coagulating and noncoagulating conditions. Blood advances, 4(13), 2953-2961.
    • Kroone, C., Vos, M., Rademakers, T., Kuijpers, M., Hoogenboezem, M., van Buul, J., Heemskerk, J. W. M., Ruf, W., van Hylckama Vlieg, A., Versteeg, H. H., Goumans, M-J., de Vries, C. J. M., Kurakula, K., & INVENT Consortium (2020). LIM-only protein FHL2 attenuates vascular tissue factor activity, inhibits thrombus formation in mice and FHL2 genetic variation associates with human venous thrombosis. Haematologica-the Hematology Journal, 105(6), 1677-1685.
    • Shi, C., Kim, T., Steiger, S., Mulay, S. R., Klinkhammer, B. M., Baeuerle, T., Melica, M. E., Romagnani, P., Moeckel, D., Baues, M., Yang, L., Brouns, S. L. N., Heemskerk, J. W. M., Braun, A., Lammers, T., Boor, P., & Anders, H-J. (2020). Crystal Clots as Therapeutic Target in Cholesterol Crystal Embolism. Circulation Research, 126(8), E37-E52.
    • Brouns, S. L. N., Provenzale, I., van Geffen, J. P., van der Meijden, P. E. J., & Heemskerk, J. W. M. (2020). Localized endothelial-based control of platelet aggregation and coagulation under flow: A proof-of-principle vessel-on-a-chip study. Journal of Thrombosis and Haemostasis, 18(4), 931-941.
    • Sternkopf, M., Nagy, M., Baaten, C. C. F. M. J., Kuijpers, M. J. E., Tullemans, B. M. E., Wirth, J., Theelen, W., Mastenbroek, T. G., Lehrke, M., Winnerling, B., Baerts, L., Marx, N., De Meester, I., Doring, Y., Cosemans, J. M. E. M., Daiber, A., Steven, S., Jankowski, J., Heemskerk, J. W. M., & Noels, H. (2020). Native, Intact Glucagon-Like Peptide 1 Is a Natural Suppressor of Thrombus Growth Under Physiological Flow Conditions. Arteriosclerosis Thrombosis and Vascular Biology, 40(3), E65-E77.
    • Onselaer, M-B., Nagy, M., Pallini, C., Pike, J. A., Perrella, G., Quintanilla, L. G., Eble, J. A., Poulter, N. S., Heemskerk, J. W. M., & Watson, S. P. (2020). Comparison of the GPVI inhibitors losartan and honokiol. Platelets, 31(2), 187-197.
  • 2019
    • Provenzale, I., Brouns, S. L. N., van der Meijden, P. E. J., Swieringa, F., & Heemskerk, J. W. M. (2019). Whole Blood Based Multiparameter Assessment of Thrombus Formation in Standard Microfluidic Devices to Proxy In Vivo Haemostasis and Thrombosis. Micromachines, 10(11), [787].
    • Coppin, L., Najimi, M., Bodart, J., Rouchon, M-S., van der Smissen, P., Eeckhoudt, S., Dahlqvist, G., Castanares-Zapatero, D., Komuta, M., Brouns, S. L., Baaten, C. C., Heemskerk, J. W. M., Horman, S., Belmonte, N., Sokal, E., & Stephenne, X. (2019). Clinical Protocol to Prevent Thrombogenic Effect of Liver-Derived Mesenchymal Cells for Cell-Based Therapies. Cells, 8(8), [846].
    • Nagy, M., van Geffen, J. P., Stegner, D., Adams, D. J., Braun, A., de Witt, S. M., Elvers, M., Geer, M. J., Kuijpers, M. J. E., Kunzelmann, K., Mori, J., Oury, C., Pircher, J., Pleines, I., Poole, A. W., Senis, Y. A., Verdoold, R., Weber, C., Nieswandt, B., ... Baaten, C. C. F. M. J. (2019). Comparative Analysis of Microfluidics Thrombus Formation in Multiple Genetically Modified Mice: Link to Thrombosis and Hemostasis. Frontiers in cardiovascular medicine, 6, [99].
    • Govers-Riemslag, J. W. P., Konings, J., Cosemans, J. M. E. M., van Geffen, J. P., de Laat, B., Heemskerk, J. W. M., Dargaud, Y., & Ten Cate, H. (2019). Impact of Deficiency of Intrinsic Coagulation Factors XI and XII on Ex Vivo Thrombus Formation and Clot Lysis. TH open : companion journal to thrombosis and haemostasis, 3(3), e273-e285.
    • Gotru, S. K., van Geffen, J. P., Nagy, M., Mammadova-Bach, E., Eilenberger, J., Volz, J., Manukjan, G., Schulze, H., Wagner, L., Eber, S., Schambeck, C., Deppermann, C., Brouns, S., Nurden, P., Greinacher, A., Sachs, U., Nieswandt, B., Hermanns, H. M., Heemskerk, J. W. M., & Braun, A. (2019). Defective Zn2+ homeostasis in mouse and human platelets with alpha- and delta-storage pool diseases. Scientific Reports, 9, [8333].
    • Jooss, N. J., De Simone, I., Provenzale, I., Fernández, D. I., Brouns, S. L. N., Farndale, R. W., Henskens, Y. M. C., Kuijpers, M. J. E., Ten Cate, H., van der Meijden, P. E. J., Cavill, R., & Heemskerk, J. W. M. (2019). Role of Platelet Glycoprotein VI and Tyrosine Kinase Syk in Thrombus Formation on Collagen-Like Surfaces. International journal of molecular sciences, 20(11), [2788].
    • van Geffen, A., Brouns, S., Batista, J., McKinney, H., Kempster, C., Nagy, M., Sivapalaratnam, S., Baaten, C., Bourry, N., Frontini, M., Jurk, K., Krause, M., Pilliteri, D., Swieringa, F., Verdoold, R., Cavill, R., Kuijpers, M., Ouwehand, W. H., Downes, K., & Heemskerk, J. (2019). High-throughput elucidation of thrombus formation reveals sources of platelet function variability. Haematologica-the Hematology Journal, 104(6), 1256-1267.
    • Slater, A., Perrella, G., Onselaer, M-B., Martin, E. M., Gauer, J. S., Xu, R-G., Heemskerk, J. W. M., Ariens, R. A. S., & Watson, S. P. (2019). Does fibrin(ogen) bind to monomeric or dimeric GPVI, or not at all?Platelets, 30(3), 281-289.
    • van der Meijden, P. E. J., & Heemskerk, J. W. M. (2019). Platelet biology and functions: new concepts and clinical perspectives. Nature Reviews Cardiology, 16(3), 166-179.
    • Mammadova-Bach, E., Nagy, M., Heemskerk, J. W. M., Nieswandt, B., & Braun, A. (2019). Store-operated calcium entry in thrombosis and thrombo-inflammation. Cell Calcium, 77, 39-48.
    • Kiouptsi, K., Jaeckel, S., Pontarollo, G., Grill, A., Kuijpers, M. J. E., Wilms, E., Weber, C., Sommer, F., Nagy, M., Neideck, C., Jansen, Y., Ascher, S., Formes, H., Karwot, C., Bayer, F., Kollar, B., Subramaniam, S., Molitor, M., Wenzel, P., ... Reinhardt, C. (2019). The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice. Mbio, 10(5), [e02298-19].
  • 2018
    • Baaten, C. C. F. M. J., Meacham, S., de Witt, S. M., Feijge, M. A. H., Adams, D. J., Akkerman, J-W. N., Cosemans, J. M. E. M., Grassi, L., Jupe, S., Kostadima, M., Mattheij, N. J. A., Prins, M. H., Ramirez-Solis, R., Soehnlein, O., Swieringa, F., Weber, C., White, J. K., Ouwehand, W. H., & Heemskerk, J. W. M. (2018). A synthesis approach of mouse studies to identify genes and proteins in arterial thrombosis and bleeding. Blood, 132(24), E35-E46.
    • Tullemans, B. M. E., Nagy, M., Sabrkhany, S., Griffioen, A. W., Egbrink, M. G. A. O., Aarts, M., Heemskerk, J. W. M., & Kuijpers, M. J. E. (2018). Tyrosine Kinase Inhibitor Pazopanib Inhibits Platelet Procoagulant Activity in Renal Cell Carcinoma Patients. Frontiers in cardiovascular medicine, 5, [142].
    • Hofmann, I., Geer, M. J., Vogtle, T., Crispin, A., Campagna, D. R., Barr, A., Calicchio, M. L., Heising, S., van Geffen, J. P., Kuijpers, M. J. E., Heemskerk, J. W. M., Eble, J. A., Schmitz-Abe, K., Obeng, E. A., Douglas, M., Freson, K., Pondarre, C., Favier, R., Jarvis, G. E., ... Senis, Y. A. (2018). Congenital macrothrombocytopenia with focal myelofibrosis due to mutations in human G6b-B is rescued in humanized mice. Blood, 132(13), 1399-1412.
    • Geer, M. J., van Geffen, J. P., Gopalasingam, P., Vogtle, T., Smith, C. W., Heising, S., Kuijpers, M. J. E., Tullemans, B. M. E., Jarvis, G. E., Eble, J. A., Jeeves, M., Overduin, M., Heemskerk, J. W. M., Mazharian, A., & Senis, Y. A. (2018). Uncoupling ITIM receptor G6b-B from tyrosine phosphatases Shp1 and Shp2 disrupts murine platelet homeostasis. Blood, 132(13), 1413-1425.
    • Baaten, C. C. F. M. J., Swieringa, F., Misztal, T., Mastenbroek, T. G., Feijge, M. A. H., Bock, P. E., Donners, M. M. P. C., Collins, P. W., Li, R., van der Meijden, P. E. J., & Heemskerk, J. W. M. (2018). Platelet heterogeneity in activation-induced glycoprotein shedding: functional effects. Blood advances, 2(18), 2320-2331.
    • Lepropre, S., Kautbally, S., Octave, M., Ginion, A., Onselaer, M-B., Steinberg, G. R., Kemp, B. E., Hego, A., Wera, O., Brouns, S., Swieringa, F., Giera, M., Darley-Usmar, V. M., Ambroise, J., Guigas, B., Heemskerk, J., Bertrand, L., Oury, C., Beauloye, C., & Horman, S. (2018). AMPK-ACC signaling modulates platelet phospholipids and potentiates thrombus formation. Blood, 132(11), 1180-1192.
    • Farmery, J. H. R., Smith, M. L., Lynch, A. G., Huissoon, A., Furnell, A., Mead, A., Levine, A. P., Manzur, A., Thrasher, A., Greenhalgh, A., Parker, A., Sanchis-Juan, A., Richter, A., Gardham, A., Lawrie, A., Sohal, A., Creaser-Myers, A., Frary, A., Greinacher, A., ... NIHR-BioResource Rare Dis (2018). Telomerecat: A ploidy-agnostic method for estimating telomere length from whole genome sequencing data. Scientific Reports, 8, [1300].
    • Tullemans, B. M. E., Heemskerk, J. W. M., & Kuijpers, M. J. E. (2018). Acquired platelet antagonism: off-target antiplatelet effects of malignancy treatment with tyrosine kinase inhibitors. Journal of Thrombosis and Haemostasis, 16(9), 1686-1699.
    • Baaten, C. C. F. M. J., Moenen, F. C. J. I., Henskens, Y. M. C., Swieringa, F., Wetzels, R. J. H., van Oerle, R., Heijnen, H. F. G., ten Cate, H., Holloway, G. P., Beckers, E. A. M., Heemskerk, J. W. M., & van der Meijden, P. E. J. (2018). Impaired mitochondrial activity explains platelet dysfunction in thrombocytopenic cancer patients undergoing chemotherapy. Haematologica-the Hematology Journal, 103(9), 1557-1567.
    • Swieringa, F., Spronk, H. M. H., Heemskerk, J. W. M., & van der Meijden, P. E. J. (2018). Integrating platelet and coagulation activation in fibrin clot formation. Research and practice in thrombosis and haemostasis, 2(3), 450-460.
    • Vajen, T., Heinzmann, A. C. A., Dickhout, A., Zhao, Z., Nagy, M., Heemskerk, J. W. M., & Koenen, R. R. (2018). Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets. Journal of visualized experiment, (134), [e57009].
    • Borst, O., Munzer, P., Alnaggar, N., Geue, S., Tegtmeyer, R., Rath, D., Droppa, M., Seizer, P., Heitmeier, S., Heemskerk, J. W. M., Jennings, L. K., Storey, R. F., Angiolillo, D. J., Rocca, B., Spronk, H., Ten Cate, H., Gawaz, M., & Geisler, T. (2018). Inhibitory mechanisms of very low-dose rivaroxaban in non-ST-elevation myocardial infarction. Blood advances, 2(6), 715-730.
    • Mori, J., Nagy, Z., Di Nunzio, G., Smith, C. W., Geer, M. J., Al Ghaithi, R., van Geffen, J. P., Heising, S., Boothman, L., Tullemans, B. M. E., Correia, J. N., Tee, L., Kuijpers, M. J. E., Harrison, P., Heemskerk, J. W. M., Jarvis, G. E., Tarakhovsky, A., Weiss, A., Mazharian, A., & Senis, Y. A. (2018). Maintenance of murine platelet homeostasis by the kinase Csk and phosphatase CD148. Blood, 131(10), 1122-1144.
    • Thomassen, S., Mastenbroek, T. G., Swieringa, F., Winckers, K., Feijge, M. A. H., Schrijver, R., Cosemans, J. M. E. M., Maroney, S. A., Mast, A. E., Hackeng, T. M., & Heemskerk, J. W. M. (2018). Suppressive Role of Tissue Factor Pathway Inhibitor-alpha in Platelet-Dependent Fibrin Formation under Flow Is Restricted to Low Procoagulant Strength. Thrombosis and Haemostasis, 118(3), 502-513.
    • Nagy, M., Mastenbroek, T. G., Mattheij, N. J. A., de Witt, S., Clemetson, K. J., Kirschner, J., Schulz, A. S., Vraetz, T., Speckmann, C., Braun, A., Cosemans, J. M. E. M., Zieger, B., & Heemskerk, J. W. M. (2018). Variable impairment of platelet functions in patients with severe, genetically linked immune deficiencies. Haematologica-the Hematology Journal, 103(3), 540-549.
    • Spronk, H. M. H., Padro, T., Siland, J. E., Prochaska, J. H., Winters, J., van der Wal, A. C., Posthuma, J. J., Lowe, G., d'Alessandro, E., Wenzel, P., Coenen, D. M., Reitsma, P. H., Ruf, W., van Gorp, R. H., Koenen, R. R., Vajen, T., Alshaikh, N. A., Wolberg, A. S., Macrae, F. L., ... ten Cate, H. (2018). Atherothrombosis and Thromboembolism: Position Paper from the Second Maastricht Consensus Conference on Thrombosis. Thrombosis and Haemostasis, 118(2), 229-250.
    • Brouns, S. L. N., van Geffen, J. P., & Heemskerk, J. W. M. (2018). High-throughput measurement of human platelet aggregation under flow: application in hemostasis and beyond. Platelets, 29(7), 662-669.
    • Loosse, C., Swieringa, F., Heemskerk, J. W. M., Sickmann, A., & Lorenz, C. (2018). Platelet proteomics: from discovery to diagnosis. Expert Review of Proteomics, 15(6), 467-476.
  • 2017
    • BioResource-Rare Dis Consortium, UK Natl Cohort Study Idiopathic He, BRIDGE-BPD Consortium, Henskens, Y., Heemskerk, J., & Vries, M. (2017). Phenotypic Characterization of EIF2AK4 Mutation Carriers in a Large Cohort of Patients Diagnosed Clinically With Pulmonary Arterial Hypertension. Circulation, 136(21), 2022-2033.
    • Baaten, C. C. F. M. J., ten Cate, H., van der Meijden, P. E. J., & Heemskerk, J. W. M. (2017). Platelet populations and priming in hematological diseases. Blood Reviews, 31(6), 389-399.
    • Petersen, R., Lambourne, J. J., Javierre, B. M., Grassi, L., Kreuzhuber, R., Ruklisa, D., Rosa, I. M., Tome, A. R., Elding, H., van Geffen, J. P., Jiang, T., Farrow, S., Cairns, J., Al-Subaie, A. M., Ashford, S., Attwood, A., Batista, J., Bouman, H., Burden, F., ... Frontini, M. (2017). Platelet function is modified by common sequence variation in megakaryocyte super enhancers. Nature Communications, 8, [16058].
    • Borstlap, W. A. A., Deijen, C. L., den Dulk, M., Bonjer, H. J., van de Velde, C. J., Bemelman, W. A., Tanis, P. J., Aalbers, A., Acherman, Y., Algie, G. D., von Geu-sau, B. A., Amelung, F., Aukema, T. S., Bakker, I. S., Bartels, S. A., Basha, S., Bastiaansen, A. J. N. M., Belgers, E., Bleeker, W., ... Dutch Snapshot Res Grp (2017). Benchmarking recent national practice in rectal cancer treatment with landmark randomized controlled trials. Colorectal Disease, 19(6), O219-O231.
    • Egan, K., van Geffen, J. P., ma, H., Kevane, B., Lennon, A., Allen, S., Neary, E., Parsons, M., Maguire, P., Wynne, K., Kennedy, R. O., Heemskerk, J. M., & Ainle, F. N. (2017). Effect of platelet-derived beta-thromboglobulins on coagulation. Thrombosis Research, 154, 7-15.
    • Vajen, T., Benedikter, B. J., Heinzmann, A. C. A., Vasina, E. M., Henskens, Y., Parsons, M., Maguire, P. B., Stassen, F. R., Heemskerk, J. W. M., Schurgers, L. J., & Koenen, R. R. (2017). Platelet extracellular vesicles induce a pro-inflammatory smooth muscle cell phenotype. Journal of Extracellular Vesicles , 6(1), [1322454].
    • von Hundelshausen, P., Agten, S. M., Eckardt, V., Blanchet, X., Schmitt, M. M., Ippel, H., Neideck, C., Bidzhekov, K., Leberzammer, J., Wichapong, K., Faussner, A., Drechsler, M., Grommes, J., van Geffen, J. P., Li, H., Ortega-Gomez, A., Megens, R. T. A., Naumann, R., Dijkgraaf, I., ... Weber, C. (2017). Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation. Science Translational Medicine, 9(384), [eaah6650].
    • Beck, F., Geiger, J., Gambaryan, S., Solari, F. A., Dell'Aica, M., Loroch, S., Mattheij, N. J., Mindukshev, I., Poetz, O., Jurk, K., Burkhart, J. M., Fufezan, C., Heemskerk, J. W. M., Walter, U., Zahedi, R. P., & Sickmann, A. (2017). Temporal quantitative phosphoproteomics of ADP stimulation reveals novel central nodes in platelet activation and inhibition. Blood, 129(2), E1-E12.
    • Carss, K. J., Arno, G., Erwood, M., Stephens, J., Sanchis-Juan, A., Hull, S., Megy, K., Grozeva, D., Dewhurst, E., Malka, S., Plagnol, V., Penkett, C., Stirrups, K., Rizzo, R., Wright, G., Josifova, D., Bitner-Glindzicz, M., Scott, R. H., Clement, E., ... Raymond, F. L. (2017). Comprehensive Rare Variant Analysis via Whole-Genome Sequencing to Determine the Molecular Pathology of Inherited Retinal Disease. American Journal of Human Genetics, 100(1), 75-90.
    • Nagy, M., Heemskerk, J. W. M., & Swieringa, F. (2017). Use of microfluidics to assess the platelet-based control of coagulation. Platelets, 28(5), 441-448.
  • 2016
    • Baig, A. A., Haining, E. J., Geuss, E., Beck, S., Swieringa, F., Wanitchakool, P., Schuhmann, M. K., Stegner, D., Kunzelmann, K., Kleinschnitz, C., Heemskerk, J. W. M., Braun, A., & Nieswandt, B. (2016). TMEM16F-Mediated Platelet Membrane Phospholipid Scrambling Is Critical for Hemostasis and Thrombosis but not Thromboinflammation in Mice: Brief Report. Arteriosclerosis Thrombosis and Vascular Biology, 36(11), 2152-2157.