Rory Koenen

Associate professor

Dr Rory  Koenen studied biochemistry in Sittard (BSc) and at the Radboud University in Nijmegen (MSc), the Netherlands from 1993-1999. He completed his PhD in cardiovascular sciences at CARIM Maastricht University in 2005. He then worked in Germany at IMCAR, RWTH Aachen University until 2010 and then moved to IPEK at the LMU in Munich chaired by Christian Weber. In 2012, he returned to CARIM as the recipient of a Vidi grant.

Dr Koenen is currently investigating the functions of platelets beyond hemostasis, platelet microvesicles and the role of particular inflammatory molecules in vascular disease e.g. thrombosis and atherosclerosis. For example, he aims to characterise the molecular aspects of chemokine RANTES and platelet factor 4 secretion from platelets and its importance in the development of cardiovascular diseases. In addition, Dr Koenen is interested in the role of cell adhesion molecules in platelet function and in how platelets maintain interactions with other cell types (leukocytes, endothelial and smooth muscle cells). Finally, Dr Koenen investigates how platelets and their microvesicles can influence the behavior and phenotype of these cells.

Department of Biochemistry
Universiteitsingel 50, 6229 ER Maastricht
PO Box 616, 6200 MD Maastricht
Room number: 4.366a
T: +31(0)43 388 33 90

  • 2020
    • Denisov, S. S., Ramirez-Escudero, M., Heinzmann, A. C. A., Ippel, J. H., Dawson, P. E., Koenen, R. R., Hackeng, T. M., Janssen, B. J. C., & Dijkgraaf, I. (2020). Structural characterization of anti-CCL5 activity of the tick salivary protein evasin-4. Journal of Biological Chemistry, 295(42), 14367-14378. https://doi.org/10.1074/jbc.RA120.013891
    • Heinzmann, A. C. A., Karel, M. F. A., Coenen, D. M., Vajen, T., Meulendijks, N. M. M., Nagy, M., Suylen, D. P. L., Cosemans, J. M. E. M., Heemskerk, J. W. M., Hackeng, T. M., & Koenen, R. R. (2020). Complementary roles of platelet alpha(IIb)beta(3) integrin, phosphatidylserine exposure and cytoskeletal rearrangement in the release of extracellular vesicles. Atherosclerosis, 310, 17-25. https://doi.org/10.1016/j.atherosclerosis.2020.07.015
    • Koenen, R. R., & Binder, C. J. (2020). Platelets and coagulation factors: Established and novel roles in atherosclerosis and atherothrombosis. Atherosclerosis, 307, 78-79. https://doi.org/10.1016/j.atherosclerosis.2020.07.008
    • Benedikter, B. J., & Koenen, R. R. (2020). Vaping, vapor, vesicles! Electronic cigarettes provoke vascular extracellular vesicle release in healthy volunteers. Atherosclerosis, 301, 79-81. https://doi.org/10.1016/j.atherosclerosis.2020.03.012
    • Denisov, S. S., Heinzmann, A. C. A., Vajen, T., Vries, M. H. M., Megens, R. T. A., Suylen, D., Koenen, R. R., Post, M. J., Ippel, J. H., Hackeng, T. M., & Dijkgraaf, I. (2020). Tick Saliva Protein Evasin-3 Allows for Visualization of Inflammation in Arteries through Interactions with CXC-Type Chemokines Deposited on Activated Endothelium. Bioconjugate Chemistry, 31(3), 948-955. https://doi.org/10.1021/acs.bioconjchem.0c00095
  • 2019
    • Denisov, S. S., Ippel, J. H., Heinzmann, A. C. A., Koenen, R. R., Ortega-Gomez, M., Soehnlein, O., Hackeng, T. M., & Dijkgraaf, I. (2019). Tick saliva protein Evasin-3 modulates chemotaxis by disrupting CXCL8 interactions with glycosaminoglycans and CXCR2. Journal of Biological Chemistry, 294(33), 12370-12379. https://doi.org/10.1074/jbc.RA119.008902
    • Koenen, R. R. (2019). Lysophosphatidylcholine in Platelet Microvesicles: The Grease for Cardiovascular Disease. Thrombosis and Haemostasis, 119(8), 1202-1204. https://doi.org/10.1055/s-0039-1693024
    • Noels, H., Weber, C., & Koenen, R. R. (2019). Chemokines as Therapeutic Targets in Cardiovascular Disease The Road Behind, The Road Ahead. Arteriosclerosis Thrombosis and Vascular Biology, 39(4), 583-592. https://doi.org/10.1161/ATVBAHA.118.312037
    • Koenen, R. R. (2019). No hearty reception: infusion of CXCL4 impedes tissue repair by macrophages after myocardial infarction. Cardiovascular Research, 115(2), 264-265. https://doi.org/10.1093/cvr/cvy241
    • Benedikter, B. J., Bouwman, F. G., Heinzmann, A. C. A., Vajen, T., Mariman, E. C., Wouters, E. F. M., Savelkoul, P. H. M., Koenen, R. R., Rohde, G. G. U., van Oerle, R., Spronk, H. M., & Stassen, F. R. M. (2019). Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles. Journal of Extracellular Vesicles , 8(1), [1585163]. https://doi.org/10.1080/20013078.2019.1585163
  • 2018
    • Koenen, R. R., & Aikawa, E. (2018). Editorial: Extracellular Vesicle-Mediated Processes in Cardiovascular Diseases. Frontiers in cardiovascular medicine, 5, [133]. https://doi.org/10.3389/fcvm.2018.00133
    • Dickhout, A., & Koenen, R. R. (2018). Extracellular Vesicles as Biomarkers in Cardiovascular Disease; Chances and Risks. Frontiers in cardiovascular medicine, 5, [113]. https://doi.org/10.3389/fcvm.2018.00113
    • Vajen, T., Koenen, R. R., Werner, I., Staudt, M., Projahn, D., Curaj, A., Sonmez, T. T., Simsekyilmaz, S., Schumacher, D., Mollmann, J., Hackeng, T. M., von Hundelshausen, P., Weber, C., & Liehn, E. A. (2018). Blocking CCL5-CXCL4 heteromerization preserves heart function after myocardial infarction by attenuating leukocyte recruitment and NETosis. Scientific Reports, 8, [10647]. https://doi.org/10.1038/s41598-018-29026-0
    • Schurgers, L. J., Akbulut, A. C., Kaczor, D. M., Halder, M., Koenen, R. R., & Kramann, R. (2018). Initiation and Propagation of Vascular Calcification Is Regulated by a Concert of Platelet- and Smooth Muscle Cell-Derived Extracellular Vesicles. Frontiers in cardiovascular medicine, 5, [36]. https://doi.org/10.3389/fcvm.2018.00036
    • Vajen, T., Heinzmann, A. C. A., Dickhout, A., Zhao, Z., Nagy, M., Heemskerk, J. W. M., & Koenen, R. R. (2018). Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets. Journal of visualized experiment, (134), [e57009]. https://doi.org/10.3791/57009
    • Spronk, H. M. H., Padro, T., Siland, J. E., Prochaska, J. H., Winters, J., van der Wal, A. C., Posthuma, J. J., Lowe, G., d'Alessandro, E., Wenzel, P., Coenen, D. M., Reitsma, P. H., Ruf, W., van Gorp, R. H., Koenen, R. R., Vajen, T., Alshaikh, N. A., Wolberg, A. S., Macrae, F. L., ... ten Cate, H. (2018). Atherothrombosis and Thromboembolism: Position Paper from the Second Maastricht Consensus Conference on Thrombosis. Thrombosis and Haemostasis, 118(2), 229-250. https://doi.org/10.1160/TH17-07-0492
    • Curaj, A., Wu, Z., Rix, A., Gresch, O., Sternkopf, M., Alampour-Rajabi, S., Lammers, T., van Zandvoort, M., Weber, C., Koenen, R. R., Liehn, E. A., & Kiessling, F. (2018). Molecular Ultrasound Imaging of Junctional Adhesion Molecule A Depicts Acute Alterations in Blood Flow and Early Endothelial Dysregulation. Arteriosclerosis Thrombosis and Vascular Biology, 38(1), 40-48. https://doi.org/10.1161/ATVBAHA.117.309503
  • 2017
    • Benedikter, B. J., Bouwman, F. G., Vajen, T., Heinzmann, A. C. A., Grauls, G., Mariman, E. C., Wouters, E. F. M., Savelkoul, P. H., Lopez-Iglesias, C., Koenen, R. R., Rohde, G. G. U., & Stassen, F. R. M. (2017). Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies. Scientific Reports, 7, [15297]. https://doi.org/10.1038/s41598-017-15717-7
    • Zhao, Z., Vajen, T., Karshovska, E., Dickhout, A., Schmitt, M. M., Megens, R. T. A., von Hundelshausen, P., Koeppel, T. A., Hackeng, T. M., Weber, C., & Koenen, R. R. (2017). Deletion of junctional adhesion molecule A from platelets increases early-stage neointima formation after wire injury in hyperlipidemic mice. Journal of Cellular and Molecular Medicine, 21(8), 1523-1531. https://doi.org/10.1111/jcmm.13083
    • Vajen, T., Benedikter, B. J., Heinzmann, A. C. A., Vasina, E. M., Henskens, Y., Parsons, M., Maguire, P. B., Stassen, F. R., Heemskerk, J. W. M., Schurgers, L. J., & Koenen, R. R. (2017). Platelet extracellular vesicles induce a pro-inflammatory smooth muscle cell phenotype. Journal of Extracellular Vesicles , 6(1), [1322454]. https://doi.org/10.1080/20013078.2017.1322454
    • von Hundelshausen, P., Agten, S. M., Eckardt, V., Blanchet, X., Schmitt, M. M., Ippel, H., Neideck, C., Bidzhekov, K., Leberzammer, J., Wichapong, K., Faussner, A., Drechsler, M., Grommes, J., van Geffen, J. P., Li, H., Ortega-Gomez, A., Megens, R. T. A., Naumann, R., Dijkgraaf, I., ... Weber, C. (2017). Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation. Science Translational Medicine, 9(384), [eaah6650]. https://doi.org/10.1126/scitranslmed.aah6650
    • Koenen, R. (2017). Extrazelluläre Vesikel im Blut: Der verlängerte Arm der Blutplättchen. Trillium Diagnostik, 15(4), 256-258. https://www.trillium.de/zeitschriften/trillium-diagnostik/trillium-diagnostik-ausgaben-2017/td-42017/schwerpunkt/extrazellulaere-vesikel-der-verlaengerte-arm-der-blutplaettchen.html?L=0
  • 2016
    • Agten, S. M., Koenen, R. R., Ippel, H., Eckardt, V., von Hundelshausen, P., Mayo, K. H., Weber, C., & Hackeng, T. M. (2016). Probing Functional Heteromeric Chemokine Protein-Protein Interactions through Conformation-Assisted Oxime Ligation. Angewandte Chemie-International Edition, 55(48), 14963-14966. https://doi.org/10.1002/anie.201607036
    • Regn, M., Laggerbauer, B., Jentzsch, C., Ramanujam, D., Ahles, A., Sichler, S., Calzada-Wack, J., Koenen, R. R., Braun, A., Nieswandt, B., & Engelhardt, S. (2016). Peptidase inhibitor 16 is a membrane-tethered regulator of chemerin processing in the myocardium. Journal of Molecular and Cellular Cardiology, 99, 57-64. https://doi.org/10.1016/j.yjmcc.2016.08.010
    • Koenen, R. R. (2016). The prowess of platelets in immunity and inflammation. Thrombosis and Haemostasis, 116(4), 605-612. https://doi.org/10.1160/TH16-04-0300
  • 2015
    • Medina, I., Cougoule, C., Drechsler, M., Bermudez, B., Koenen, R. R., Sluimer, J., Wolfs, I., Doering, Y., Herias, V., Gijbels, M., Bot, I., de Jager, S. C. A., Weber, C., Cleutjens, J., van Berkel, T. J. C., Sikkink, K-J., Mocsai, A., Maridonneau-Parini, I., Soehnlein, O., & Biessen, E. A. L. (2015). Hck/Fgr Kinase Deficiency Reduces Plaque Growth and Stability by Blunting Monocyte Recruitment and Intraplaque Motility. Circulation, 132(6), 490-501. https://doi.org/10.1161/CIRCULATIONAHA.114.012316
    • Vajen, T., Mause, S. F., & Koenen, R. R. (2015). Microvesicles from platelets: novel drivers of vascular inflammation. Thrombosis and Haemostasis, 114(2), 228-236. https://doi.org/10.1160/TH14-11-0962
    • Karshovska, E., Zhao, Z., Blanchet, X., Schmitt, M. M. N., Bidzhekov, K., Soehnlein, O., von Hundelshausen, P., Mattheij, N. J., Cosemans, J. M. E. M., Megens, R. T. A., Koeppel, T. A., Schober, A., Hackeng, T. M., Weber, C., & Koenen, R. R. (2015). Hyperreactivity of Junctional Adhesion Molecule A-Deficient Platelets Accelerates Atherosclerosis in Hyperlipidemic Mice. Circulation Research, 116(4), 587-599. https://doi.org/10.1161/CIRCRESAHA.116.304035
  • 2014
    • Blanchet, X., Cesarek, K., Brandt, J., Herwald, H., Teupser, D., Kuechenhoff, H., Karshovska, E., Mause, S. F., Siess, W., Wasmuth, H., Soehnlein, O., Koenen, R. R., Weber, C., & von Hundelshausen, P. (2014). Inflammatory role and prognostic value of platelet chemokines in acute coronary syndrome. Thrombosis and Haemostasis, 112(6), 1277-1287. https://doi.org/10.1160/TH14-02-0139
    • Bot, I., Daissormont, I. T. M. N., Zernecke, A., van Puijvelde, G. H. M., Kramp, B., de Jager, S. C. A., Sluimer, J. C., Manca, M., Herias, V., Westra, M. M., Bot, M., van Santbrink, P. J., van Berkel, T. J. C., Su, L., Skjelland, M., Gullestad, L., Kuiper, J., Halvorsen, B., Aukrust, P., ... Biessen, E. A. L. (2014). CXCR4 blockade induces atherosclerosis by affecting neutrophil function. Journal of Molecular and Cellular Cardiology, 74, 44-52. https://doi.org/10.1016/j.yjmcc.2014.04.021
    • Schmitt, M. M. N., Fraemohs, L., Hackeng, T. M., Weber, C., & Koenen, R. R. (2014). Atherogenic mononuclear cell recruitment is facilitated by oxidized lipoprotein-induced endothelial junctional adhesion molecule-A redistribution. Atherosclerosis, 234(2), 254-264. https://doi.org/10.1016/j.atherosclerosis.2014.03.014
    • Projahn, D., Simsekyilmaz, S., Singh, S., Kanzler, I., Kramp, B. K., Langer, M., Burlacu, A., Bernhagen, J., Klee, D., Zernecke, A., Hackeng, T. M., Groll, J., Weber, C., Liehn, E. A., & Koenen, R. R. (2014). Controlled intramyocardial release of engineered chemokines by biodegradable hydrogels as a treatment approach of myocardial infarction. Journal of Cellular and Molecular Medicine, 18(5), 790-800. https://doi.org/10.1111/jcmm.12225
    • Koenen, R. R. (2014). CX(3)CL1, a special deputy at the intersection of platelets and the vessel wall. Thrombosis and Haemostasis, 111(4), 567-567. https://doi.org/10.1160/TH14-02-0173
    • Li, X., Zhu, M., Penfold, M. E., Koenen, R. R., Thiemann, A., Heyll, K., Akhtar, S., Koyadan, S., Wu, Z., Gremse, F., Kiessling, F., van Zandvoort, M., Schall, T. J., Weber, C., & Schober, A. (2014). Activation of CXCR7 Limits Atherosclerosis and Improves Hyperlipidemia by Increasing Cholesterol Uptake in Adipose Tissue. Circulation, 129(11), 1244-1253. https://doi.org/10.1161/CIRCULATIONAHA.113.006840
    • Tilstam, P. V., Gijbels, M. J., Habbeddine, M., Cudejko, C., Asare, Y., Theelen, W., Zhou, B., Doering, Y., Drechsler, M., Pawig, L., Simsekyilmaz, S., Koenen, R. R., de Winther, M. P. J., Lawrence, T., Bernhagen, J., Zernecke, A., Weber, C., & Noels, H. (2014). Bone Marrow-Specific Knock-In of a Non-Activatable Ikk alpha Kinase Mutant Influences Haematopoiesis but Not Atherosclerosis in Apoe-Deficient Mice. PLOS ONE, 9(2), [e87452]. https://doi.org/10.1371/journal.pone.0087452
    • Jaczewska, J., Abdulreda, M. H., Yau, C. Y., Schmitt, M. M., Schubert, I., Berggren, P-O., Weber, C., Koenen, R. R., Moy, V. T., & Wojcikiewicz, E. P. (2014). TNF-alpha and IFN-gamma promote lymphocyte adhesion to endothelial junctional regions facilitating transendothelial migration. Journal of Leukocyte Biology, 95(2), 265-274. https://doi.org/10.1189/jlb.0412205
    • Schmitt, M. M. N., Megens, R. T. A., Zernecke, A., Bidzhekov, K., van den Akker, N. M., Rademakers, T., van Zandvoort, M. A., Hackeng, T. M., Koenen, R. R., & Weber, C. (2014). Endothelial Junctional Adhesion Molecule-A Guides Monocytes Into Flow-Dependent Predilection Sites of Atherosclerosis. Circulation, 129(1), 66-+. https://doi.org/10.1161/CIRCULATIONAHA.113.004149
  • 2013
    • Agten, S. M., Suylen, D., Ippel, H., Kokozidou, M., Tans, G., van de Vijver, P., Koenen, R. R., & Hackeng, T. M. (2013). Chemoselective Oxime Reactions in Proteins and Peptides by Using an Optimized Oxime Strategy: The Demise of Levulinic Acid. Chembiochem, 14(18), 2431-2434. https://doi.org/10.1002/cbic.201300598
    • Kramp, B. K., Megens, R. T. A., Sarabi, A., Winkler, S., Projahn, D., Weber, C., Koenen, R. R., & von Hundelshausen, P. (2013). Exchange of extracellular domains of CCR1 and CCR5 reveals confined functions in CCL5-mediated cell recruitment. Thrombosis and Haemostasis, 110(4), 795-806. https://doi.org/10.1160/TH13-05-0420
    • Soehnlein, O., Drechsler, M., Doering, Y., Lievens, D., Hartwig, H., Kemmerich, K., Ortega-Gomez, A., Mandl, M., Vijayan, S., Projahn, D., Garlichs, C. D., Koenen, R. R., Hristov, M., Lutgens, E., Zernecke, A., & Weber, C. (2013). Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes. EMBO Molecular Medicine, 5(3), 471-481. https://doi.org/10.1002/emmm.201201717
  • 2012
    • Projahn, D., & Koenen, R. R. (2012). Platelets: key players in vascular inflammation. Journal of Leukocyte Biology, 92(6), 1167-1175. https://doi.org/10.1189/jlb.0312151
    • Van de Vijver, P., Schmitt, M., Suylen, D., Scheer, L., Thomassen, M. C. L. G. D., Schurgers, L. J., Griffin, J. H., Koenen, R. R., & Hackeng, T. M. (2012). Incorporation of Disulfide Containing Protein Modules into Multivalent Antigenic Conjugates: Generation of Antibodies against the Thrombin-Sensitive Region of Murine Protein S. Journal of the American Chemical Society, 134(47), 19318-19321. https://doi.org/10.1021/ja306993t
    • Nazari-Jahantigh, M., Wei, Y., Noels, H., Akhtar, S., Zhou, Z., Koenen, R. R., Heyll, K., Gremse, F., Kiessling, F., Grommes, J., Weber, C., & Schober, A. (2012). MicroRNA-155 promotes atherosclerosis by repressing Bcl6 in macrophages. Journal of Clinical Investigation, 122(11), 4190-4202. https://doi.org/10.1172/JCI61716
    • Doering, Y., Soehnlein, O., Drechsler, M., Shagdarsuren, E., Chaudhari, S. M., Meiler, S., Hartwig, H., Hristov, M., Koenen, R. R., Hieronymus, T., Zenke, M., Weber, C., & Zernecke, A. (2012). Hematopoietic Interferon Regulatory Factor 8-Deficiency Accelerates Atherosclerosis in Mice. Arteriosclerosis Thrombosis and Vascular Biology, 32(7), 1613-1623. https://doi.org/10.1161/ATVBAHA.111.236539
    • Postea, O., Vasina, E. M., Cauwenberghs, S., Projahn, D., Liehn, E. A., Lievens, D., Theelen, W., Kramp, B. K., Butoi, E. D., Soehnlein, O., Heemskerk, J. W. M., Ludwig, A., Weber, C., & Koenen, R. R. (2012). Contribution of Platelet CX(3)CR1 to Platelet-Monocyte Complex Formation and Vascular Recruitment During Hyperlipidemia. Arteriosclerosis Thrombosis and Vascular Biology, 32(5), 1186-+. https://doi.org/10.1161/ATVBAHA.111.243485
    • Doering, Y., Manthey, H. D., Drechsler, M., Lievens, D., Megens, R. T. A., Soehnlein, O., Busch, M., Manca, M., Koenen, R. R., Pelisek, J., Daemen, M. J., Lutgens, E., Zenke, M., Binder, C. J., Weber, C., & Zernecke, A. (2012). Auto-Antigenic Protein-DNA Complexes Stimulate Plasmacytoid Dendritic Cells to Promote Atherosclerosis. Circulation, 125(13), 1673-U190. https://doi.org/10.1161/CIRCULATIONAHA.111.046755
    • Grommes, J., Alard, J-E., Drechsler, M., Wantha, S., Morgelin, M., Kuebler, W. M., Jacobs, M., von Hundelshausen, P., Markart, P., Wygrecka, M., Preissner, K. T., Hackeng, T. M., Koenen, R. R., Weber, C., & Soehnlein, O. (2012). Disruption of Platelet-derived Chemokine Heteromers Prevents Neutrophil Extravasation in Acute Lung Injury. American Journal of Respiratory and Critical Care Medicine, 185(6), 628-636. https://doi.org/10.1164/rccm.201108-1533OC
    • Kanzler, I., Liehn, E. A., Koenen, R. R., & Weber, C. (2012). Anti-Inflammatory Therapeutic Approaches to Reduce Acute Atherosclerotic Complications. Current Pharmaceutical Biotechnology, 13(1), 37-45. https://doi.org/10.2174/138920112798868557