PhD candidate Biochemistry department14-09-2021
PhD candidate for Department of Biochemistry
The project aims to develop and work on a stem cell-based vessel-on-a-chip model to investigate thrombosis. This will focus on hPSC differentiations towards endothelial and vascular smooth muscle cells, CRISPR-Cas9 gene editing and integration into dynamic flow systems for translational modeling.
The cause of thrombosis lies within the three pillars based on Virchows’ Triad: vessel, flow, and blood. Traditionally these three pillars are approached separately by research. This project will utilize world leading expertise of these three pillars to combine them into development of a novel stem cell model, Virchows’ Organoid. The project will involve pluripotent stem cell culture, vascular cell differentiation (endothelial and vascular smooth muscle cell), CRISPRCas9 gene editing and flow-mechanics to build a model to study the three pillars of Virchows’ Triad. This interdisciplinary approach will allow novel understanding of coagulation and thrombosis by combining vessel wall (imaging and RNA-sequencing), flow dynamics (platelet flow) and changes to blood protein biochemistry (ELISA) in a single model, termed Virchows’ Organoid.
The successful applicant will be placed in the Department of Biochemistry, CARIM in our newly established pluripotent stem cell facility under guidance of Prof. Leon Schurgers (vessels).